The Detection of in Vivo and in Vitro HIV Type 1 B/F Profiles in Brazil Using a Real-Time PCR Assay for Five HIV Type 1 Genomic Regions

Show simple item record

dc.contributor.author Teixeira, Daniela [UNIFESP]
dc.contributor.author Munerato, Patricia [UNIFESP]
dc.contributor.author Vasconcelos Komninakis, Shirley Cavalcante [UNIFESP]
dc.contributor.author Fusuma, Erika Etsuko [UNIFESP]
dc.contributor.author Janini, Luiz Mario [UNIFESP]
dc.contributor.author Araripe Sucupira, Maria Cecilia [UNIFESP]
dc.contributor.author Diaz, Ricardo Sobhie [UNIFESP]
dc.date.accessioned 2016-01-24T14:05:26Z
dc.date.available 2016-01-24T14:05:26Z
dc.date.issued 2010-09-01
dc.identifier http://dx.doi.org/10.1089/aid.2010.0023
dc.identifier.citation Aids Research and Human Retroviruses. New Rochelle: Mary Ann Liebert Inc, v. 26, n. 9, p. 981-990, 2010.
dc.identifier.issn 0889-2229
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32889
dc.description.abstract We sought to determine the frequency and profile of HIV-1 BF recombinants in vitro and in vivo. Laboratory HIV-1 strains from subtypes B and F were cocultured and evaluated. Clinical samples from the city of Santos, Brazil, where the first HIV-1 B/F circulating recombinant forms (CRF) were described, were also assessed. Five real-time PCR assays were developed to equally amplify subtypes B and F, and subtype-specific probes were developed and optimized. To validate the PCR systems, clinical samples from Santos were sequenced and phylogenetically analyzed. the real-time PCR assays were performed on these samples and on the supernatant of an in vitro competition assay to assess emergent recombinant strains. Out of 157 clinical samples, 62.1% were defined as subtype B, 3.0% were subtype F, 16.7% presented the CRF28_BF profile, and 13.6% of the samples presented the CRF29_BF profile. the specificity and sensitivity in the discrimination assay for this sample panel were 93% and 92%, respectively. the HIV that emerged from the coinfected cell culture closely resembled the CRF28_BF profile. the first-described CRFs are still fixed in this geographic region of Brazil, and the in vitro emerging strains detected by real-time PCR suggest that in addition to the shaping of recombinant strains by immune selection, viral structures may also play an important role in emerging CRFs. en
dc.description.sponsorship Department of STD/AIDS of the Ministry of Health
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 981-990
dc.language.iso eng
dc.publisher Mary Ann Liebert Inc
dc.relation.ispartof Aids Research and Human Retroviruses
dc.rights Acesso restrito
dc.title The Detection of in Vivo and in Vitro HIV Type 1 B/F Profiles in Brazil Using a Real-Time PCR Assay for Five HIV Type 1 Genomic Regions en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Paulista Sch Med, Retrovirol Lab, BR-04039032 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Paulista Sch Med, Retrovirol Lab, BR-04039032 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 04/15856-9
dc.description.sponsorshipID FAPESP: 06/52722-6
dc.identifier.doi 10.1089/aid.2010.0023
dc.description.source Web of Science
dc.identifier.wos WOS:000281510000007



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account