Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells

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dc.contributor.author Santos, G. A. dos
dc.contributor.author Thomé, Carolina Hassibe [UNIFESP]
dc.contributor.author Ferreira, Germano A
dc.contributor.author Yoneda, Juliana Sakamoto
dc.contributor.author Nobre, Thatyane Morimoto
dc.contributor.author Perez, Katia Regina [UNIFESP]
dc.contributor.author Scheucher, Priscila Santos
dc.contributor.author Gimenes-Teixeira, Hamilton Luiz
dc.contributor.author Constantino, Maurício Gomes
dc.contributor.author Oliveira, Kleber Thiago de
dc.contributor.author Faça, Vítor Marcel
dc.contributor.author Falcão, Roberto Passetto
dc.contributor.author Greene, Lewis Joel [UNIFESP]
dc.contributor.author Rego, Eduardo Magalhães
dc.contributor.author Ciancaglini, Pietro
dc.date.accessioned 2016-01-24T14:05:21Z
dc.date.available 2016-01-24T14:05:21Z
dc.date.issued 2010-09-01
dc.identifier http://dx.doi.org/10.1016/j.bbamem.2010.05.013
dc.identifier.citation Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1798, n. 9, p. 1714-1723, 2010.
dc.identifier.issn 0005-2736
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32834
dc.description.abstract 10-(Octyloxy) decyl-2-(trimethylammonium) ethyl phosphate (ODPC) is an alkylphospholipid that can interact with cell membranes because of its amphiphilic character. We describe here the interaction of ODPC with liposomes and its toxicity to leukemic cells with an ED-50 of 5.4, 5.6 and 2.9 pM for 72 h of treatment for inhibition of proliferation of NB4, U937 and K562 cell lines, respectively, and lack of toxicity to normal hematopoietic progenitor cells at concentrations up to 25 pM. the ED-50 for the non-malignant HEK-293 and primary human umbilical vein endothelial cells (HUVEC) was 63.4 and 60.7 mu M, respectively. the critical micellar concentration (CMC) of ODPC was 200 mu M. Dynamic light scattering indicated that dipalmitoylphosphatidylcholine (DPPC) liposome size was affected only above the CMC of ODPC. Differential calorimetric scanning (DCS) of liposomes indicated a critical transition temperature (T(c)) of 41.5 degrees C and an enthalpy (Delta H) variation of 7.3 kcal mol(-1). the presence of 25 mu M ODPC decreased T(c) and Delta H to 393 degrees C and 4.7 kcal mol(-1), respectively. ODPC at 250 mu M destabilized the liposomes (36.3 degrees C. 0.46 kcal mol(-1)). Kinetics of 5(6)-carboxyfluorescein (CF) leakage from different liposome systems indicated that the rate and extent of CF release depended on liposome composition and ODPC concentration and that above the CMC it was instantaneous. Overall, the data indicate that ODPC acts on in vitro membrane systems and leukemia cell lines at concentrations below its CMC, suggesting that it does not act as a detergent and that this effect is dependent on membrane composition. (C) 2010 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Fundacao de Apoio ao Ensino
dc.description.sponsorship Pesquisa e Assistencia do Hospital das Clinicas da FMRP-USP (FAEPA)
dc.format.extent 1714-1723
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Biochimica Et Biophysica Acta-biomembranes
dc.rights Acesso aberto
dc.subject 10-(Octyloxy) decyl-2-(trimethylammonium) en
dc.subject ethyl phosphate en
dc.subject Lipids en
dc.subject Liposome en
dc.subject Alkylphospholipids en
dc.subject Leukemia en
dc.title Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Inst Nacl Ciencia & Tecnol Celulas Tronco & Terap
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal de São Carlos (UFSCar)
dc.description.affiliation USP, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliation Inst Nacl Ciencia & Tecnol Celulas Tronco & Terap, São Paulo, Brazil
dc.description.affiliation USP, Fac Med Ribeirao Preto, Dept Clin Med, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04039002 São Paulo, Brazil
dc.description.affiliation USP, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliation USP, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, Brazil
dc.description.affiliation Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04039002 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2008/06619-4
dc.identifier.file WOS000280752100009.pdf
dc.identifier.doi 10.1016/j.bbamem.2010.05.013
dc.description.source Web of Science
dc.identifier.wos WOS:000280752100009



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