Number of expressed cancer/testis antigens identifies focal adhesion pathway genes as possible targets for multiple myeloma therapy

Number of expressed cancer/testis antigens identifies focal adhesion pathway genes as possible targets for multiple myeloma therapy

Author Andrade, Valeria C. C. Google Scholar
Vettore, Andre L. Autor UNIFESP Google Scholar
Panepucci, Rodrigo A. Google Scholar
Almeida, Manuella S. S. Google Scholar
Yamamoto, Mihoko Google Scholar
De Carvalho, Fabricio Google Scholar
Caballero, Otavia L. Google Scholar
Zago, Marco Antonio Google Scholar
Colleoni, Gisele W. B. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Ludwig Inst Canc Res
Abstract Considering that the importance of cancer/testis (CT) antigens in multiple myeloma (MM) biology is still under investigation, the present study aimed to: (1) identify genes differentially expressed in MM using microarray analysis of plasma cell samples, separated according to the number of expressed CTs; (2) examine possible pathways related to MM pathogenesis; (3) validate the expression of candidate genes by quantitative real-time PCR (RQ-PCR). Three samples predominantly positive (>6 expressed), including the U266 cell line, and three samples predominantly negative (0 or 1 expressed CT for the 13 analyzed CT antigens), were submitted for microarray analysis. Validation by RQ-PCR from 24 MM samples showed that the ITGAS gene was downregulated in predominantly positive (>6 expressed CTs, p = 0.0030) and in tumor versus normal plasma cells (p = 0.0182). the RhoD gene was overexpressed in tumor plasma cells when compared to normal plasma cells (p = 0.0339). Results of the microarray analysis corroborate the hypothesis that MM could be separated into predominantly positive and predominantly negative expression. the differential expression of ITGA5 and RhoD suggests disruption of the focal adhesion pathway in MM and offers a new target field to be explored in this disease.
Keywords Multiple myeloma
RhoD gene
ITGA5 gene
cancer/testis antigens
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04/13213-3
FAPESP: 04/12855-1
Date 2010-08-01
Published in Leukemia & Lymphoma. Abingdon: Taylor & Francis Ltd, v. 51, n. 8, p. 1543-1549, 2010.
ISSN 1042-8194 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 1543-1549
Origin http://dx.doi.org/10.3109/10428194.2010.491136
Access rights Closed access
Type Article
Web of Science ID WOS:000281639200028
URI http://repositorio.unifesp.br/handle/11600/32814

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