Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Author Oliveira Junior, Clovis J. F. Google Scholar
Cavalari, Aline A. Autor UNIFESP Google Scholar
Carpita, Nicholas C. Google Scholar
Buckeridge, Marcos S. Google Scholar
Braga, Marcia R. Google Scholar
Institution Inst Bot
Universidade Federal de São Paulo (UNIFESP)
Purdue Univ
Universidade de São Paulo (USP)
Abstract Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans, xylogalacturonans and arabinogalactans to be the predominant EP. in the cell wall, homogalacturonans and arabinogalactans are the major pectins, and xyloglucans and xylans are the major cross-linking glycans. the presence of xylogalacturonans in the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. the xyloglucan from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cell-wall xyloglucans.
Keywords Atlantic Forest
Cell wall polysaccharides
Rubiaceae
Xylogalacturonans
Arabinoxylan
Xyloglucan
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 98/05124-8
FAPESP: 05/04139-7
Date 2010-08-01
Published in Trees-structure and Function. New York: Springer, v. 24, n. 4, p. 713-722, 2010.
ISSN 0931-1890 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 713-722
Origin http://dx.doi.org/10.1007/s00468-010-0441-x
Access rights Closed access
Type Article
Web of Science ID WOS:000280090500012
URI http://repositorio.unifesp.br/handle/11600/32770

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