Soybean and fish oil mixture increases IL-10, protects against DNA damage and decreases colonic inflammation in rats with dextran sulfate sodium (DSS) colitis

Soybean and fish oil mixture increases IL-10, protects against DNA damage and decreases colonic inflammation in rats with dextran sulfate sodium (DSS) colitis

Author Barros, Karina V. Autor UNIFESP Google Scholar
Xavier, Roberta A. N. Autor UNIFESP Google Scholar
Abreu, Gilclay G. Autor UNIFESP Google Scholar
Martinez, Carlos A. R. Google Scholar
Ribeiro, Marcelo L. Google Scholar
Gambero, Alessandra Google Scholar
Carvalho, Patricia O. Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Silveira, Vera L. F. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Sao Francisco
Abstract It was investigated whether dietary polyunsaturated fatty acids (PUFA) could influence colonic injury, tissue DNA damage, cytokines and myeloperoxidase activity (MPO) and plasma corticosterone in DSS-induced colitis rats. Male weaning Wistar rats were fed for 47 days with an AIN-93 diet with control (C), fish (F) or a mixture of fish and soybean oil (SF). the colitis was induced from day 36 until day 42 by 3% DSS in drinking water. On day 48, blood samples were collected for corticosterone determination. the distal colon was excised for histological analysis and to quantify the cytokine (IL-4, IL-10 and INF-gamma), MPO and DNA damage. the disease activity index (DAI) was recorded daily during colitis induction. the DAI, MPO, histological analyses showed decreases only in the SF group compared with the C group. IL-10 was increased and DNA damage was reduced in the groups F and SF, and an inverse correlation between these variables was found. There were no differences in corticosterone, IFN-gamma and IL-4 levels. Soybean and fish oil mixture may be effective in improving colonic injury and DNA damage, and it could be an important complementary therapy in UC to reduce the use of anti-inflammatory drugs and prevent colorectal cancer.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-07-08
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 9, 9 p., 2010.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 9
Origin http://dx.doi.org/10.1186/1476-511X-9-68
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000280405200001
URI http://repositorio.unifesp.br/handle/11600/32722

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