Obesity induced by high-fat diet promotes insulin resistance in the ovary

Show simple item record

dc.contributor.author Akamine, Eliana H.
dc.contributor.author Marcal, Anderson C.
dc.contributor.author Camporez, Joao Paulo
dc.contributor.author Hoshida, Mara S.
dc.contributor.author Caperuto, Luciana C. [UNIFESP]
dc.contributor.author Bevilacqua, Estela
dc.contributor.author Carvalho, Carla R. O.
dc.date.accessioned 2016-01-24T14:05:05Z
dc.date.available 2016-01-24T14:05:05Z
dc.date.issued 2010-07-01
dc.identifier http://dx.doi.org/10.1677/JOE-09-0461
dc.identifier.citation Journal of Endocrinology. Bristol: Bioscientifica Ltd, v. 206, n. 1, p. 65-74, 2010.
dc.identifier.issn 0022-0795
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32658
dc.description.abstract Besides the effects on peripheral energy homeostasis, insulin also has an important role in ovarian function. Obesity has a negative effect on fertility, and may play a role in the development of the polycystic ovary syndrome in susceptible women. Since insulin resistance in the ovary could contribute to the impairment of reproductive function in obese women, we evaluated insulin signaling in the ovary of high-fat diet-induced obese rats. Female Wistar rats were submitted to a high-fat diet for 120 or 180 days, and the insulin signaling pathway in the ovary was evaluated by immunoprecipitation and immunoblotting. At the end of the diet period, we observed insulin resistance, hyperinsulinemia, an increase in progesterone serum levels, an extended estrus cycle, and altered ovarian morphology in obese female rats. Moreover, in female obese rats treated for 120 days with the high-fat diet, the increase in progesterone levels occurred together with enhancement of LH levels. the ovary from high-fat-fed female rats showed a reduction in the insulin receptor substrate/phosphatidylinositol 3-kinase/AKT intracellular pathway, associated with an increase in FOXO3a, IL1B, and TNF alpha protein expression. These changes in the insulin signaling pathway may have a role in the infertile state associated with obesity. Journal of Endocrinology (2010) 206, 65-74 en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship EHA
dc.format.extent 65-74
dc.language.iso eng
dc.publisher Bioscientifica Ltd
dc.relation.ispartof Journal of Endocrinology
dc.rights Acesso aberto
dc.title Obesity induced by high-fat diet promotes insulin resistance in the ovary en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Cell & Dev Biol, BR-05508900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biol Sci, BR-04023900 Diadema, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biol Sci, BR-04023900 Diadema, SP, Brazil
dc.description.sponsorshipID FAPESP: 2006/52163-7
dc.description.sponsorshipID FAPESP: 2006/60215-7
dc.description.sponsorshipID CNPq: 477906/2006-0
dc.identifier.doi 10.1677/JOE-09-0461
dc.description.source Web of Science
dc.identifier.wos WOS:000278873600007



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account