Obesity induced by high-fat diet promotes insulin resistance in the ovary

Obesity induced by high-fat diet promotes insulin resistance in the ovary

Author Akamine, Eliana H. Google Scholar
Marcal, Anderson C. Google Scholar
Camporez, Joao Paulo Google Scholar
Hoshida, Mara S. Google Scholar
Caperuto, Luciana C. Autor UNIFESP Google Scholar
Bevilacqua, Estela Google Scholar
Carvalho, Carla R. O. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Besides the effects on peripheral energy homeostasis, insulin also has an important role in ovarian function. Obesity has a negative effect on fertility, and may play a role in the development of the polycystic ovary syndrome in susceptible women. Since insulin resistance in the ovary could contribute to the impairment of reproductive function in obese women, we evaluated insulin signaling in the ovary of high-fat diet-induced obese rats. Female Wistar rats were submitted to a high-fat diet for 120 or 180 days, and the insulin signaling pathway in the ovary was evaluated by immunoprecipitation and immunoblotting. At the end of the diet period, we observed insulin resistance, hyperinsulinemia, an increase in progesterone serum levels, an extended estrus cycle, and altered ovarian morphology in obese female rats. Moreover, in female obese rats treated for 120 days with the high-fat diet, the increase in progesterone levels occurred together with enhancement of LH levels. the ovary from high-fat-fed female rats showed a reduction in the insulin receptor substrate/phosphatidylinositol 3-kinase/AKT intracellular pathway, associated with an increase in FOXO3a, IL1B, and TNF alpha protein expression. These changes in the insulin signaling pathway may have a role in the infertile state associated with obesity. Journal of Endocrinology (2010) 206, 65-74
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2006/52163-7
FAPESP: 2006/60215-7
CNPq: 477906/2006-0
Date 2010-07-01
Published in Journal of Endocrinology. Bristol: Bioscientifica Ltd, v. 206, n. 1, p. 65-74, 2010.
ISSN 0022-0795 (Sherpa/Romeo, impact factor)
Publisher Bioscientifica Ltd
Extent 65-74
Origin http://dx.doi.org/10.1677/JOE-09-0461
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000278873600007
URI http://repositorio.unifesp.br/handle/11600/32658

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