High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7) levels in the adipose tissue

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dc.contributor.author Coelho, Michella Soares
dc.contributor.author Lopes, Karen Lucasechi
dc.contributor.author Freitas, Raphael de Aquino
dc.contributor.author Oliveira-Sales, Elizabeth Barbosa de
dc.contributor.author Bergasmaschi, Cassia Toledo [UNIFESP]
dc.contributor.author Campos, Ruy Ribeiro
dc.contributor.author Casarini, Dulce Elena [UNIFESP]
dc.contributor.author Carmona, Adriana Karaoglanovic [UNIFESP]
dc.contributor.author Araujo, Mariana da Silva [UNIFESP]
dc.contributor.author Heimann, Joel Claudio
dc.contributor.author Dolnikoff, Miriam Sterman [UNIFESP]
dc.date.accessioned 2016-01-24T13:59:48Z
dc.date.available 2016-01-24T13:59:48Z
dc.date.issued 2010-06-08
dc.identifier http://dx.doi.org/10.1016/j.regpep.2010.03.008
dc.identifier.citation Regulatory Peptides. Amsterdam: Elsevier B.V., v. 162, n. 1-3, p. 61-67, 2010.
dc.identifier.issn 0167-0115
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32628
dc.description.abstract Sucrose-fed rats, a model of metabolic syndrome, are characterized by insulin resistance, obesity, hypertension, and high plasma levels of triacylglycerols and angiotensin II (Ang II). However, whether tissue renin-angiotensin system (RAS) is altered in metabolic syndrome is unclear. To study this issue, food ad libitum and water (C) or 20% sucrose solution (SC) were given to adult male Wistar rats, for 30 days. Body weight (BW), blood pressure (BP), epididymal adipose tissue (EPI) mass, rate of in vivo fatty acid (FA) synthesis in EPI, circulating glucose, insulin, leptin, angiotensins I and II, triacylglycerols, and plasma renin (PRA) and angiotensin-converting enzyme (ACE) activities were evaluated. in kidneys and EPI, gene and protein expression of type 1 (AT(1)) and 2 (AT(2)) Ang II receptors, ACE, angiotensinogen (ACT) as well as protein expression of angiotensin-converting enzyme 2 (ACE2) were determined. in both tissues, Ang I, Ang II and Ang-(1-7) contents were also measured by HPLC. in SC rats higher BP, EPI mass, circulating triacylglycerols, insulin, leptin, PRA and, Ang II were found. in EPI, the rate of in vivo FA synthesis was associated with increased Ang-(1-7), protein expression of AT(1) and AT(2) receptors, ACE2, ACT, and gene expression of ACT although a reduction in ACE activity and in adipose Ang I and Ang II contents was observed. in kidneys, AT(1) and AT(2), ACE and ACT gene and protein expression as well as protein expression of ACE2 were unaltered while Ang II, Ang-(1-7) and ACE activity increased. These RAS component changes seem to be tissue specific and possibly are related to enhancement of FA synthesis, EPI mass and hypertension. (C) 2010 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 61-67
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Regulatory Peptides
dc.rights Acesso restrito
dc.subject Sucrose feeding en
dc.subject Fatty acid synthesis en
dc.subject Renin-angiotensin system en
dc.subject ACE2 en
dc.subject Angiotensin-(1-7) en
dc.subject AT(1) and AT(2) receptors en
dc.title High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7) levels in the adipose tissue en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Fisiol, Disciplina Fisiol Cardiovasc, Escola Paulista Med, BR-04026060 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Med, Lab Expt Hypertens, Dept Internal Med, BR-05508 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Hlth Sci, BR-04026060 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Div Renal, Dept Internal Med, BR-04026060 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biophys, BR-04026060 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, BR-04026060 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Fisiol, Disciplina Fisiol Cardiovasc, Escola Paulista Med, BR-04026060 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Hlth Sci, BR-04026060 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Div Renal, Dept Internal Med, BR-04026060 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biophys, BR-04026060 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, BR-04026060 São Paulo, Brazil
dc.identifier.doi 10.1016/j.regpep.2010.03.008
dc.description.source Web of Science
dc.identifier.wos WOS:000278663300009



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