High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7) levels in the adipose tissue

High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7) levels in the adipose tissue

Author Coelho, Michella Soares Google Scholar
Lopes, Karen Lucasechi Google Scholar
Freitas, Raphael de Aquino Google Scholar
Oliveira-Sales, Elizabeth Barbosa de Google Scholar
Bergasmaschi, Cassia Toledo Autor UNIFESP Google Scholar
Campos, Ruy Ribeiro Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Carmona, Adriana Karaoglanovic Autor UNIFESP Google Scholar
Araujo, Mariana da Silva Autor UNIFESP Google Scholar
Heimann, Joel Claudio Google Scholar
Dolnikoff, Miriam Sterman Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Sucrose-fed rats, a model of metabolic syndrome, are characterized by insulin resistance, obesity, hypertension, and high plasma levels of triacylglycerols and angiotensin II (Ang II). However, whether tissue renin-angiotensin system (RAS) is altered in metabolic syndrome is unclear. To study this issue, food ad libitum and water (C) or 20% sucrose solution (SC) were given to adult male Wistar rats, for 30 days. Body weight (BW), blood pressure (BP), epididymal adipose tissue (EPI) mass, rate of in vivo fatty acid (FA) synthesis in EPI, circulating glucose, insulin, leptin, angiotensins I and II, triacylglycerols, and plasma renin (PRA) and angiotensin-converting enzyme (ACE) activities were evaluated. in kidneys and EPI, gene and protein expression of type 1 (AT(1)) and 2 (AT(2)) Ang II receptors, ACE, angiotensinogen (ACT) as well as protein expression of angiotensin-converting enzyme 2 (ACE2) were determined. in both tissues, Ang I, Ang II and Ang-(1-7) contents were also measured by HPLC. in SC rats higher BP, EPI mass, circulating triacylglycerols, insulin, leptin, PRA and, Ang II were found. in EPI, the rate of in vivo FA synthesis was associated with increased Ang-(1-7), protein expression of AT(1) and AT(2) receptors, ACE2, ACT, and gene expression of ACT although a reduction in ACE activity and in adipose Ang I and Ang II contents was observed. in kidneys, AT(1) and AT(2), ACE and ACT gene and protein expression as well as protein expression of ACE2 were unaltered while Ang II, Ang-(1-7) and ACE activity increased. These RAS component changes seem to be tissue specific and possibly are related to enhancement of FA synthesis, EPI mass and hypertension. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Sucrose feeding
Fatty acid synthesis
Renin-angiotensin system
AT(1) and AT(2) receptors
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-06-08
Published in Regulatory Peptides. Amsterdam: Elsevier B.V., v. 162, n. 1-3, p. 61-67, 2010.
ISSN 0167-0115 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 61-67
Origin http://dx.doi.org/10.1016/j.regpep.2010.03.008
Access rights Closed access
Type Article
Web of Science ID WOS:000278663300009
URI http://repositorio.unifesp.br/handle/11600/32628

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