Insulin-like growth factor binding protein-3 gene methylation and protein expression in gastric adenocarcinoma

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dc.contributor.author Gigek, Carolina Oliveira
dc.contributor.author Leal, Mariana Ferreira
dc.contributor.author Frias Lisboa, Luara Carolina
dc.contributor.author Oliveira Silva, Patricia Natalia
dc.contributor.author Chen, Elizabeth Suchi
dc.contributor.author Lima, Eleonidas Moura
dc.contributor.author Calcagno, Danielle Queiroz
dc.contributor.author Assumpcao, Paulo Pimentel
dc.contributor.author Burbano, Rommel Rodriguez
dc.contributor.author Cardoso Smith, Marilia de Arruda [UNIFESP]
dc.date.accessioned 2016-01-24T13:59:45Z
dc.date.available 2016-01-24T13:59:45Z
dc.date.issued 2010-06-01
dc.identifier http://dx.doi.org/10.1016/j.ghir.2010.02.005
dc.identifier.citation Growth Hormone & Igf Research. Edinburgh: Churchill Livingstone, v. 20, n. 3, p. 234-238, 2010.
dc.identifier.issn 1096-6374
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32591
dc.description.abstract Objective: the aim of this study was to evaluate IGFBP-3 protein expression, its correlation with gene promoter methylation pattern in gastric carcinogenesis and with clinicopathological characteristics.Design: Forty-three normal gastric mucosa and 94 adenocarcinoma samples were investigated through methylation specific PCR, after bisulfite modification. Immunohistochemistry was analyzed using peroxidase in 54 gastric cancer and 20 normal gastric mucosa samples.Results: IGFBP-3 expression was higher in tumor samples than in normal mucosa (p < 0.0001). Intestinal type presented a higher frequency of protein expression than diffuse type (p = 0.0412). Methylation frequency of IGFBP-3 promoter in gastric samples revealed, respectively, 95.7% and 97.7% in neoplastic and non-neoplastic samples. the frequency of IGFBP-3 methylation did not differ between tumor and normal samples (95.7% versus 97.7%, p = 0.7810). We did not observe a significant correlation between IGFBP-3 promoter methylation and protein expression.Conclusion: in summary, our study did not observe any influence of IGFBP-3 promoter methylation on protein expression. Moreover we propose that IGFBP-3 immunostaining in gastric tissue may be a useful marker for malignancy. (C) 2010 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 234-238
dc.language.iso eng
dc.publisher Churchill Livingstone
dc.relation.ispartof Growth Hormone & Igf Research
dc.rights Acesso restrito
dc.subject Gastric cancer en
dc.subject IGFBP-3 en
dc.subject Methylation en
dc.subject Protein expression en
dc.title Insulin-like growth factor binding protein-3 gene methylation and protein expression in gastric adenocarcinoma en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Fed Piaui
dc.contributor.institution Fed Univ Para
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliation Univ Fed Piaui, Dept Biol, Teresina, PI, Brazil
dc.description.affiliation Fed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66059 Belem, Para, Brazil
dc.description.affiliation Fed Univ Para, Hosp Univ Joao de Barros Barreto, Serv Cirurgia, BR-66059 Belem, Para, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1016/j.ghir.2010.02.005
dc.description.source Web of Science
dc.identifier.wos WOS:000278652100009



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