Insulin-like growth factor binding protein-3 gene methylation and protein expression in gastric adenocarcinoma

Insulin-like growth factor binding protein-3 gene methylation and protein expression in gastric adenocarcinoma

Author Gigek, Carolina Oliveira Google Scholar
Leal, Mariana Ferreira Google Scholar
Frias Lisboa, Luara Carolina Google Scholar
Oliveira Silva, Patricia Natalia Google Scholar
Chen, Elizabeth Suchi Google Scholar
Lima, Eleonidas Moura Google Scholar
Calcagno, Danielle Queiroz Google Scholar
Assumpcao, Paulo Pimentel Google Scholar
Burbano, Rommel Rodriguez Google Scholar
Cardoso Smith, Marilia de Arruda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Piaui
Fed Univ Para
Abstract Objective: the aim of this study was to evaluate IGFBP-3 protein expression, its correlation with gene promoter methylation pattern in gastric carcinogenesis and with clinicopathological characteristics.Design: Forty-three normal gastric mucosa and 94 adenocarcinoma samples were investigated through methylation specific PCR, after bisulfite modification. Immunohistochemistry was analyzed using peroxidase in 54 gastric cancer and 20 normal gastric mucosa samples.Results: IGFBP-3 expression was higher in tumor samples than in normal mucosa (p < 0.0001). Intestinal type presented a higher frequency of protein expression than diffuse type (p = 0.0412). Methylation frequency of IGFBP-3 promoter in gastric samples revealed, respectively, 95.7% and 97.7% in neoplastic and non-neoplastic samples. the frequency of IGFBP-3 methylation did not differ between tumor and normal samples (95.7% versus 97.7%, p = 0.7810). We did not observe a significant correlation between IGFBP-3 promoter methylation and protein expression.Conclusion: in summary, our study did not observe any influence of IGFBP-3 promoter methylation on protein expression. Moreover we propose that IGFBP-3 immunostaining in gastric tissue may be a useful marker for malignancy. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Gastric cancer
Protein expression
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2010-06-01
Published in Growth Hormone & Igf Research. Edinburgh: Churchill Livingstone, v. 20, n. 3, p. 234-238, 2010.
ISSN 1096-6374 (Sherpa/Romeo, impact factor)
Publisher Churchill Livingstone
Extent 234-238
Access rights Closed access
Type Article
Web of Science ID WOS:000278652100009

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