Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR, TEM and Z-scan technique

Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR, TEM and Z-scan technique

Author Gomez, S. L. Google Scholar
Monteiro, A. M. Google Scholar
Rabbani, S. R. Google Scholar
Bloise, A. C. Google Scholar
Carneiro, S. M. Google Scholar
Alves, S. Autor UNIFESP Google Scholar
Gidlund, M. Google Scholar
Abdalla, D. S. P. Google Scholar
Figueiredo Neto, A. M. Google Scholar
Institution Univ Estadual Ponta Grossa
Universidade de São Paulo (USP)
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Abstract In this work we report on a study of the morphological changes of LDL induced in vitro by metallic ions (Cu(2+) and Fe(3+)). These modifications were characterized by transmission electron microscopy, nuclear magnetic resonance and the Z-scan technique. the degree of oxidative modification of LDL was determined by the TBARS and lipid hydroperoxides assays. It is shown that distinct pathways for modifying lipoproteins lead to different morphological transformations of the particles characterized by changes in size and/or shape of the resulting particles, and by the tendency to induce aggregation of the particles. There were no evidence of melting of particles promoted by oxidative processes with Cu and Fe. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Keywords oxLDL
Lipoprotein
NMR
TEM
Z-scan technique
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
National Institute of Science and Technology of Complex Fluids (INCT-FCx)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-06-01
Published in Chemistry and Physics of Lipids. Clare: Elsevier B.V., v. 163, n. 6, p. 545-551, 2010.
ISSN 0009-3084 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 545-551
Origin http://dx.doi.org/10.1016/j.chemphyslip.2010.03.008
Access rights Closed access
Type Article
Web of Science ID WOS:000280033500012
URI http://repositorio.unifesp.br/handle/11600/32587

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