APOA1/A5 Variants and Haplotypes as a Risk Factor for Obesity and Better Lipid Profiles in a Brazilian Elderly Cohort

APOA1/A5 Variants and Haplotypes as a Risk Factor for Obesity and Better Lipid Profiles in a Brazilian Elderly Cohort

Author Chen, Elizabeth Suchi Autor UNIFESP Google Scholar
Furuya, Tatiane Katsue Autor UNIFESP Google Scholar
Mazzotti, Diego Robles Autor UNIFESP Google Scholar
Ota, Vanessa Kiyomi Autor UNIFESP Google Scholar
Cendoroglo, Maysa Seabra Autor UNIFESP Google Scholar
Ramos, Luiz Roberto Autor UNIFESP Google Scholar
Araujo, Lara Quirino Autor UNIFESP Google Scholar
Burbano, Rommel Rodriguez Autor UNIFESP Google Scholar
Cardoso Smith, Marilia de Arruda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Genetic variations in the APOA1/C3/A4/A5 gene cluster have been studied and proposed to be the leading key for susceptibility to cardiovascular diseases and age-associated disorders. We aimed to investigate the associations of rs12721026 (APOA1) and rs1729408 (APOA5) polymorphisms and their haplotypes with some age-related diseases, as well as with lipids and proteins serum levels in a cohort from a Brazilian Elderly Longitudinal Study (EPIDOSO). Genotyping was performed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Statistical analyses were carried out using logistic regression analysis, Student's t-test, and linkage disequilibrium (LD) analysis. Polymorphic allele frequencies were 0.095 and 0.449 for rs12721026 and rs1729408, respectively. the C-allele of rs1729408 was associated with higher high-density lipoprotein (HDL) (P = 0.022) and glycated hemoglobin levels (P = 0.020). We also showed that rs12721026 and rs1729408 were in LD. the GC haplotype, which is composed of the G-allele of rs12721026 and the C-allele of rs1729408, was significantly associated with obesity (P = 0.028), with higher glycated hemoglobin (P = 0.006), and fasting glucose (P = 0.0003) compared to the TT haplotype, which includes the wild-type alleles of both polymorphisms. Moreover, we found an association between the TC haplotype and higher HDL levels (P = 0.0039). This is the first time that haplotypes involving these polymorphisms were evaluated. Our results showed that these polymorphisms were involved in the development of obesity and in alterations of lipids and proteins serum levels in a Brazilian population. the present findings might also clarify the role of these polymorphisms and their haplotypes in lipids and proteins metabolism.
Keywords Obesity
Glycated hemoglobin
Polymorphisms
APOA1
APOA5
HDL
Haplotype
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-06-01
Published in Lipids. Heidelberg: Springer Heidelberg, v. 45, n. 6, p. 511-517, 2010.
ISSN 0024-4201 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 511-517
Origin http://dx.doi.org/10.1007/s11745-010-3426-z
Access rights Closed access
Type Article
Web of Science ID WOS:000279694900005
URI http://repositorio.unifesp.br/handle/11600/32584

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