Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa

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dc.contributor.author Fogaca, Andrea C.
dc.contributor.author Zaini, Paulo A.
dc.contributor.author Wulff, Nelson A.
dc.contributor.author Silva, Patricia I. P. da
dc.contributor.author Fazio, Marcos A. [UNIFESP]
dc.contributor.author Miranda, Antonio [UNIFESP]
dc.contributor.author Daffre, Sirlei
dc.contributor.author Silva, Aline M. da
dc.date.accessioned 2016-01-24T13:59:37Z
dc.date.available 2016-01-24T13:59:37Z
dc.date.issued 2010-05-01
dc.identifier http://dx.doi.org/10.1111/j.1574-6968.2010.01950.x
dc.identifier.citation Fems Microbiology Letters. Malden: Wiley-Blackwell, v. 306, n. 2, p. 152-159, 2010.
dc.identifier.issn 0378-1097
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32487
dc.description.abstract In the xylem vessels of susceptible hosts, such as citrus trees, Xylella fastidiosa forms biofilm-like colonies that can block water transport, which appears to correlate to disease symptoms. Besides aiding host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMPs). Here, we show that gomesin, a potent AMP from a tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min of treatment with a sublethal concentration. DNA microarray hybridizations revealed that among the upregulated coding sequences, some are related to biofilm production. in addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by nontreated cells or cells exposed to streptomycin. We have also observed that the treatment of X. fastidiosa with a sublethal concentration of gomesin before inoculation in tobacco plants correlates with a reduction in foliar symptoms, an effect possibly due to the trapping of bacterial cells to fewer xylem vessels, given the enhancement in biofilm production. These results warrant further investigation of how X. fastidiosa would respond to the AMPs produced by citrus endophytes and by the insect vector, leading to a better understanding of the mechanism of action of these molecules on bacterial virulence. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 152-159
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Fems Microbiology Letters
dc.rights Acesso aberto
dc.subject antimicrobial en
dc.subject phytopathogen en
dc.subject microarray en
dc.subject gene expression profile en
dc.title Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Fundo Defesa Citricultura
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, BR-05508000 São Paulo, Brazil
dc.description.affiliation Fundo Defesa Citricultura, Araraquara, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Parasitol, Inst Ciencias Biomed, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.identifier.doi 10.1111/j.1574-6968.2010.01950.x
dc.description.source Web of Science
dc.identifier.wos WOS:000276791400010



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