Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa

Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa

Author Fogaca, Andrea C. Google Scholar
Zaini, Paulo A. Google Scholar
Wulff, Nelson A. Google Scholar
Silva, Patricia I. P. da Google Scholar
Fazio, Marcos A. Autor UNIFESP Google Scholar
Miranda, Antonio Autor UNIFESP Google Scholar
Daffre, Sirlei Google Scholar
Silva, Aline M. da Google Scholar
Institution Universidade de São Paulo (USP)
Fundo Defesa Citricultura
Universidade Federal de São Paulo (UNIFESP)
Abstract In the xylem vessels of susceptible hosts, such as citrus trees, Xylella fastidiosa forms biofilm-like colonies that can block water transport, which appears to correlate to disease symptoms. Besides aiding host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMPs). Here, we show that gomesin, a potent AMP from a tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min of treatment with a sublethal concentration. DNA microarray hybridizations revealed that among the upregulated coding sequences, some are related to biofilm production. in addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by nontreated cells or cells exposed to streptomycin. We have also observed that the treatment of X. fastidiosa with a sublethal concentration of gomesin before inoculation in tobacco plants correlates with a reduction in foliar symptoms, an effect possibly due to the trapping of bacterial cells to fewer xylem vessels, given the enhancement in biofilm production. These results warrant further investigation of how X. fastidiosa would respond to the AMPs produced by citrus endophytes and by the insect vector, leading to a better understanding of the mechanism of action of these molecules on bacterial virulence.
Keywords antimicrobial
phytopathogen
microarray
gene expression profile
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-05-01
Published in Fems Microbiology Letters. Malden: Wiley-Blackwell, v. 306, n. 2, p. 152-159, 2010.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 152-159
Origin http://dx.doi.org/10.1111/j.1574-6968.2010.01950.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000276791400010
URI http://repositorio.unifesp.br/handle/11600/32487

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