Microinjection of GABAergic agents into the anterior nucleus of the thalamus modulates pilocarpine-induced seizures and status epilepticus

Microinjection of GABAergic agents into the anterior nucleus of the thalamus modulates pilocarpine-induced seizures and status epilepticus

Autor Bittencourt, Simone Autor UNIFESP Google Scholar
Dubiela, Francisco Paulino Autor UNIFESP Google Scholar
Queiroz, Claudio Marcos Autor UNIFESP Google Scholar
Covolan, Luciene Autor UNIFESP Google Scholar
Andrade, Danielle Google Scholar
Lozano, Andres Google Scholar
Mello, Luiz Eugenio Araujo de Moraes Autor UNIFESP Google Scholar
Hamani, Clement Autor UNIFESP Google Scholar
Instituição Toronto Western Hosp
Universidade Federal de São Paulo (UNIFESP)
Resumo The anterior nucleus of the thalamus (AN) has been suggested as a potential target for seizure modulation in animal models and patients with refractory epilepsy. We investigate whether microinjections of GABAergic agonists into the AN were protective against pilocarpine-induced generalized seizures and status epilepticus (SE). Rats were treated with bilateral AN injections of muscimol (160 or 80 nmol), bicuculline (15 nmol), or saline (controls) 20 min prior to pilocarpine administration (350 mg/kg i.p.). Electrographic recordings were used to confirm seizure activity. We found that pretreatment with AN muscimol 160 nmol increased the latency to seizures and SE by 2.5-3.0-fold. This dose however was associated with side effects, particularly hypotonia. AN bicuculline was proconvulsant, whereas no major effect was observed after muscimol 80 nmol injections. the percentage of animals that developed SE was similar across groups. Overall, microinjection of high doses of muscimol into the AN delayed the occurrence of pilocarpine-induced seizures and SE but was not able to prevent these events. (C) 2010 British Epilepsy Association. Published by Elsevier B.V. All rights reserved.
Palavra-chave Thalamus
Pilocarpine
GABA
Anterior nucleus
Epilepsy
Muscimol
Deep brain stimulation
Idioma Inglês
Data de publicação 2010-05-01
Publicado em Seizure-european Journal of Epilepsy. London: W B Saunders Co Ltd, v. 19, n. 4, p. 242-246, 2010.
ISSN 1059-1311 (Sherpa/Romeo, fator de impacto)
Publicador W B Saunders Co Ltd
Extensão 242-246
Fonte http://dx.doi.org/10.1016/j.seizure.2010.02.010
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000277878700008
Endereço permanente http://repositorio.unifesp.br/handle/11600/32484

Exibir registro completo




Arquivo

Nome: WOS000277878700008.pdf
Tamanho: 340.8KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta