Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii

Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii

Author Moreira, Leandro M. Google Scholar
Almeida, Nalvo F. Google Scholar
Potnis, Neha Google Scholar
Digiampietri, Luciano A. Google Scholar
Adi, Said S. Google Scholar
Bortolossi, Julio C. Google Scholar
Silva, Ana C. da Google Scholar
Silva, Aline M. da Google Scholar
Moraes, Fabricio E. de Google Scholar
Oliveira, Julio C. de Autor UNIFESP Google Scholar
Souza, Robson F. de Google Scholar
Facincani, Agda P. Google Scholar
Ferraz, Andre L. Google Scholar
Ferro, Maria I. Google Scholar
Furlan, Luiz R. Google Scholar
Gimenez, Daniele F. Google Scholar
Jones, Jeffrey B. Google Scholar
Kitajima, Elliot W. Google Scholar
Laia, Marcelo L. Google Scholar
Leite, Rui P. Google Scholar
Nishiyama, Milton Y. Google Scholar
Neto, Julio Rodrigues Google Scholar
Nociti, Leticia A. Google Scholar
Norman, David J. Google Scholar
Ostroski, Eric H. Google Scholar
Pereira, Haroldo A. Google Scholar
Staskawicz, Brian J. Google Scholar
Tezza, Renata I. Google Scholar
Ferro, Jesus A. Google Scholar
Vinatzer, Boris A. Google Scholar
Setubal, Joao Carlos Google Scholar
Institution Virginia Polytech Inst & State Univ
Univ Fed Ouro Preto
Universidade de São Paulo (USP)
Univ Florida
Univ Estadual Paulista
Universidade Federal de São Paulo (UNIFESP)
Univ Estado Santa Catarina
Allelyx Appl Genom
Inst Biol Campinas
Universidade Federal de Mato Grosso do Sul (UFMS)
Universidade Estadual de Campinas (UNICAMP)
Inst Agron Parana
Univ Calif Berkeley
Abstract Background: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. the three types have different phenotypes and affect different citrus species. the causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C.Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. in addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein.Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. the gained knowledge will be instrumental for improving citrus canker control.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2010-04-13
Published in Bmc Genomics. London: Biomed Central Ltd, v. 11, 25 p., 2010.
ISSN 1471-2164 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 25
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000279860700001

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