Effects of Pentoxifylline on Inflammation and Lung Dysfunction in Ventilated Septic Animals

Effects of Pentoxifylline on Inflammation and Lung Dysfunction in Ventilated Septic Animals

Author Oliveira-Junior, Itamar Souza Autor UNIFESP Google Scholar
Souza Oliveira, Wagner Rogerio Autor UNIFESP Google Scholar
Cavassani, Samia Santos Autor UNIFESP Google Scholar
Colo Brunialti, Milena Karina Google Scholar
Salomao, Reinaldo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Acute respiratory distress syndrome secondary to sepsis is associated with high morbidity and mortality. the purpose of this study was to characterize the effects of ventilatory strategy and the modulating activity of pentoxifylline in a sepsis-induced lung dysfunction model. Male Wistar rats were randomly divided into six groups, undergoing two different ventilatory strategies. Rats received live Escherichia coli or saline intraperitoneally. After 6 hours, the septic animals were treated with either pentoxifylline (25 mg/kg for 20 minutes) or normal saline infusion and ventilated with low tidal volume (6 mL/kg; septic animals with E. coli intraperitoneal [IP] infusion, PTX-treated and ventilated with low tidal volume and septic animals with E. coli IP infusion and ventilated with low tidal volume, respectively) or high tidal volume (12 mL/kg; septic animals with E. coli IP infusion, PTX-treated and ventilated with high tidal volume and septic animals with E. coli IP infusion and ventilated with high tidal volume, respectively) for 3 hours. the control animals received normal saline infusion and, after 6 hours, were ventilated with low or high tidal volume (control animals with saline infusion and ventilated with low tidal volume and control animals with saline infusion and ventilated with high tidal volume, respectively). Lung dysfunctions were assessed by wet-to-dry lung ratios, total cell count, total protein, malondialdehyde, and tumor necrosis factor-alpha concentrations in bronchoalveolar lavage (BAL). Septic animals with E. coli IP infusion and ventilated with high tidal volume presented increased wet-to-dry lung ratios, total cell count, total protein, and malondialdehyde in BAL compared with the septic animals ventilated with low tidal volume. Septic animals treated with pentoxifylline presented higher arterial oxygenation and lower cellular influx, protein leakage, malondialdehyde concentration, and tumor necrosis factor-alpha levels in BAL compared with septic animals undergoing the same ventilatory support strategies (septic animals with E. coli IP infusion and ventilated with low tidal volume and septic animals with E. coli IP infusion and ventilated with high tidal volume). Ventilatory strategy modulated the inflammatory response and pulmonary alterations in a sepsis-induced acute lung injury model, and these effects are improved by pentoxifylline.
Keywords Lung injury
Sepsis
Low tidal volume
High tidal volume
Pentoxifylline
TNF-alpha
Inflammatory response
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado São Paulo
Grant number Fundacao de Amparo a Pesquisa do Estado São Paulo: 03/14098-0
Date 2010-04-01
Published in Journal of Trauma-injury Infection and Critical Care. Philadelphia: Lippincott Williams & Wilkins, v. 68, n. 4, p. 822-826, 2010.
ISSN 0022-5282 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 822-826
Origin http://dx.doi.org/10.1097/TA.0b013e3181a5f4b5
Access rights Closed access
Type Article
Web of Science ID WOS:000276663100014
URI http://repositorio.unifesp.br/handle/11600/32446

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