A new mistletoe Phthirusa pyrifolia leaf lectin with antimicrobial properties

A new mistletoe Phthirusa pyrifolia leaf lectin with antimicrobial properties

Autor Costa, Romero M. P. B. Google Scholar
Vaz, Antonio F. M. Google Scholar
Oliva, Maria L. V. Autor UNIFESP Google Scholar
Coelho, Luana C. B. B. Google Scholar
Correia, Maria T. S. Google Scholar
Carneiro-da-Cunha, Maria G. Google Scholar
Instituição Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Resumo Phthirusa pyrifolia leaf lectin (PpyLL) was obtained from the hemi-parasitic medicinal plant mistletoe through saline saturation and two consecutive chromatographic steps on Sephadex G-100 and ion exchange on CM-cellulose. SDS-PAGE of the protein under non-reducing conditions revealed a monomeric protein with a molecular weight of 15.6 kDa. Under reducing conditions in the presence of 2-mercaptoethanol, the protein showed two bands with molecular weights of 15 and 7 kDa. PpyLL, an acidic glycoprotein with 19% sugar content, was not dependent on divalent cations. It was stable up to 70 degrees C and exhibited maximum hemagglutination at pH 7.5. Lectin fluorescence emission spectra at different temperatures showed that the lectin fluorescence increased when the temperature increased. PpyLL showed a unique affinity for the phosphate derivative of fructose, fructose-1-6-biphosphate. PpyLL showed effective antimicrobial activities against bacteria (Gram-positive: Staphylococcus epidermidis. Streptococcus faecalis and Bacillus subtilis; Gram-negative: Klebsiella pneumoniae) and fungi (Fusarium lateritium and Rhizoctonia solani). Therefore, PpyLL specificity, as determined by a new sugar affinity, may be significant to determine its biological potential. (C) 2009 Elsevier B.V. All rights reserved.
Palavra-chave Lectin
Phthirusa pyrifolia
Mistletoe
Chromatography
Fluorescence
Antimicrobial activity
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Número do financiamento CAPES: 1415/2007
Data de publicação 2010-04-01
Publicado em Process Biochemistry. Oxford: Elsevier B.V., v. 45, n. 4, p. 526-533, 2010.
ISSN 1359-5113 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 526-533
Fonte http://dx.doi.org/10.1016/j.procbio.2009.11.013
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000276376900015
Endereço permanente http://repositorio.unifesp.br/handle/11600/32433

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