Akt pathway activation and increased neuropeptide Y mRNA expression in the rat hippocampus: Implications for seizure blockade

Akt pathway activation and increased neuropeptide Y mRNA expression in the rat hippocampus: Implications for seizure blockade

Author Goto, Eduardo M. Autor UNIFESP Google Scholar
Silva, Marcelo de Paula Google Scholar
Perosa, Sandra R. Autor UNIFESP Google Scholar
Arganaraz, Gustavo A. Autor UNIFESP Google Scholar
Pesquero, Joao B. Autor UNIFESP Google Scholar
Cavalheiro, Esper A. Autor UNIFESP Google Scholar
Naffah-Mazzacoratti, Maria G. Autor UNIFESP Google Scholar
Teixeira, Vicente P. C. Autor UNIFESP Google Scholar
Silva, Jose A. Google Scholar
Institution Univ Nove de Julho
Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to analyze the expression of survival-related molecules such Akt and integrin-linked kinase (ILK) to evaluate Akt pathway activation in epileptogenesis process. Furthermore, was also investigated the mRNA expression of neuropeptide Y, a considered antiepileptic neuropeptide, in the pilocarpine-induced epilepsy. Male Wistar rats were submitted to the pilocarpine model of epilepsy. Hippocampi were removed 6 h (acute phase), 12 h (late acute), 5d (silent) and 60d (chronic) after status epilepticus (SE) onset, and from animals that received pilocarpine but did not develop SE (partial group). Hippocampi collected were used to specify mRNA expression using Real-Time PCR. Immunohistochemistry assay was employed to place ILK distribution in the hippocampus and Western blot technique was used to determine Akt activation level. A decrease in ILK mRNA content was found during acute (0.39 +/- 0.03) and chronic (0.48 +/- 0.06) periods when compared to control group (0.87 +/- 0.10). Protein levels of ILK were also diminished during both periods. Partial group showed increased ILK mRNA expression (0.80 +/- 0.06) when compared with animals in the acute stage. Silent group had ILK mRNA and immunoreactivity similar to control group. Western blot assay showed an augmentation in Akt activation in silent period (0.52 +/- 0.03) in comparison with control group (0.44 +/- 0.01). Neuropeptide Y mRNA expression increased in the partial group (1.67 +/- 0.22) and in the silent phase (1.45 +/- 0.29) when compared to control group (0.36 +/- 0.12). Results suggest that neuropeptide Y (as anticonvulsant) might act in protective mechanisms occurred during epileptic phenomena. Together with ILK expression and Akt activation, these molecules could be involved in hippocampal neuroprotection in epilepsy. (C) 2009 Elsevier B.V. All rights reserved.
Keywords Integrin-linked kinase
Akt
Neuropeptide Y
Pilocarpine epilepsy model
Molecular biology
Language English
Date 2010-04-01
Published in Neuropeptides. Edinburgh: Churchill Livingstone, v. 44, n. 2, p. 169-176, 2010.
ISSN 0143-4179 (Sherpa/Romeo, impact factor)
Publisher Churchill Livingstone
Extent 169-176
Origin http://dx.doi.org/10.1016/j.npep.2009.12.007
Access rights Closed access
Type Article
Web of Science ID WOS:000276001700016
URI http://repositorio.unifesp.br/handle/11600/32429

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