Antimicrobial compounds produced by Lactobacillus sakei subsp sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product

Antimicrobial compounds produced by Lactobacillus sakei subsp sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product

Autor Carvalho, Katia G. de Google Scholar
Bambirra, Felipe H. S. Google Scholar
Kruger, Monika F. Google Scholar
Barbosa, Matheus S. Google Scholar
Oliveira, Jamil S. Google Scholar
Santos, Alexandre M. C. Google Scholar
Nicoli, Jacques R. Google Scholar
Bemquerer, Marcelo P. Google Scholar
Miranda, Antonio de Autor UNIFESP Google Scholar
Salvucci, Emiliano J. Google Scholar
Sesma, Fernando J. M. Google Scholar
Franco, Bernadette D. G. M. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de Minas Gerais (UFMG)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal de São Paulo (UNIFESP)
Ctr Referencia Lactobacilos CERELA
Univ Fed Espirito Santo
Resumo Bacteriocins produced by lactic acid bacteria are gaining increased importance due to their activity against undesirable microorganisms in foods. in this study, a concentrated acid extract of a culture of Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian pork product, was purified by cation exchange and reversed-phase chromatographic methods. the amino acid sequences of the active antimicrobial compounds determined by Edman degradation were compared to known protein sequences using the BLAST-P software. Three different antimicrobial compounds were obtained, P1, P2 and P3, and mass spectrometry indicated molecular masses of 4.4, 6.8 and 9.5 kDa, respectively. P1 corresponds to classical sakacin P, P2 is identical to the 30S ribosomal protein S21 of L. sakei subsp. sakei 23 K, and P3 is identical to a histone-like DNA-binding protein HV produced by L. sakei subsp. sakei 23 K. Total genomic DNA was extracted and used as target DNA for PCR amplification of the genes sak, lis and his involved in the synthesis of P1, P2 and P3. the fragments were cloned in pET28b expression vector and the resulting plasmids transformed in E. coli KRX competent cells. the transformants were active against Listeria monocytogenes, indicating that the activity of the classical sakacin P produced by L. sakei 2a can be complemented by other antimicrobial proteins.
Palavra-chave Lactobacillus sakei 2a
Bacteriocins
Antimicrobial proteins
Lactic acid bacteria
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Número do financiamento FAPESP: 2004/08041-9
FAPESP: 2005/60619-8
FAPEMIG: EDT 24.000
Data de publicação 2010-04-01
Publicado em Journal of Industrial Microbiology & Biotechnology. Heidelberg: Springer Heidelberg, v. 37, n. 4, p. 381-390, 2010.
ISSN 1367-5435 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 381-390
Fonte http://dx.doi.org/10.1007/s10295-009-0684-y
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000276722100007
Endereço permanente http://repositorio.unifesp.br/handle/11600/32412

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