Phosphoproteome Reveals an Atlas of Protein Signaling Networks During Osteoblast Adhesion

Phosphoproteome Reveals an Atlas of Protein Signaling Networks During Osteoblast Adhesion

Autor Milani, Renato Google Scholar
Ferreira, Carmen V. Google Scholar
Granjeiro, Jose M. Google Scholar
Paredes-Gamero, Edgar J. Autor UNIFESP Google Scholar
Silva, Rodrigo A. Google Scholar
Justo, Giselle Z. Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Galembeck, Eduardo Google Scholar
Peppelenbosch, Maikel P. Google Scholar
Aoyama, Hiroshi Google Scholar
Zambuzzi, Willian F. Google Scholar
Instituição Universidade Estadual de Campinas (UNICAMP)
Universidade Federal Fluminense (UFF)
Universidade Federal de São Paulo (UNIFESP)
Univ Groningen
Resumo Cell adhesion on surfaces is a fundamental process in the emerging biomaterials field and developmental events as well. However, the mechanisms regulating this biological process in osteoblasts are not fully understood. Reversible phosphorylation catalyzed by kinases is probably the most important regulatory mechanism in eukaryotes. Therefore, the goal of this study is to assess osteoblast adhesion through a molecular prism under a peptide array technology, revealing essential signaling proteins governing adhesion-related events. First, we showed that there are main morphological changes on osteoblast shape during adhesion up to 3 h. Second, besides classical proteins activated upon integrin activation, our results showed a novel network involving signaling proteins such as Rap1A, PKA, PKC, and GSK3 beta during osteoblast adhesion on polystyrene. Third, these proteins were grouped in different signaling cascades including focal adhesion establishment, cytoskeleton rearrangement, and cell-cycle arrest. We have thus provided evidence that a global phosphorylation screening is able to yield a systems-oriented look at osteoblast adhesion, providing new insights for understanding of bone formation and improvement of cell-substratum interactions. Altogether, these statements are necessary means for further intervention and development of new approaches for the progress of tissue engineering. Cell. Biochem. 109: 957-966, 2010. (C) 2010 Wiley-Liss, Inc.
Palavra-chave OSTEOBLAST
CELL ADHESION
SIGNAL TRANSDUCTION
SURFACES
KINOME
PKC
RAP1
BIOENGINEERING
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento FAPESP: 08/53003-9
Data de publicação 2010-04-01
Publicado em Journal of Cellular Biochemistry. Hoboken: Wiley-liss, v. 109, n. 5, p. 957-966, 2010.
ISSN 0730-2312 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 957-966
Fonte http://dx.doi.org/10.1002/jcb.22479
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000276418900015
Endereço permanente http://repositorio.unifesp.br/handle/11600/32399

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