Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma

Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma

Autor Boldarine, Valter Tadeu Autor UNIFESP Google Scholar
Maciel, Rui Monteiro de Barros Autor UNIFESP Google Scholar
Guimaraes, Gustavo Stuani Autor UNIFESP Google Scholar
Nakabashi, Claudia Cristina Doimo Autor UNIFESP Google Scholar
Camacho, Cléber Pinto Autor UNIFESP Google Scholar
Andreoni, Danielle Macellaro Autor UNIFESP Google Scholar
Mamone, Maria da Conceição de Oliveira Carneiro Autor UNIFESP Google Scholar
Ikejiri, Elza Setsuko Autor UNIFESP Google Scholar
Kasamatsu, Teresa Sayoko Autor UNIFESP Google Scholar
Crispim, Felipe Autor UNIFESP Google Scholar
Hojaij, Flavio Carneiro Autor UNIFESP Google Scholar
Hidal, Jairo Tabacow Autor UNIFESP Google Scholar
Biscolla, Rosa Paula Mello Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Israelita Ensino & Pesquisa Albert Einstein
Fleury Med & Hlth
Resumo Context: Serum thyroglobulin is a sensitive tumor marker in the follow-up of patients with differentiated thyroid carcinoma (DTC), but the presence of endogenous anti-thyroglobulin antibodies (TgAb) can interfere on its measurement. To prevent interference by TgAb, several investigators have tried to quantify blood mRNA Tg by real-time RT-PCR, but the results have been variable, not reporting a correlation between mRNA Tg and the presence of metastases.Objective: the aim of the study was to evaluate the development of a sensitive and specific quantitative RT-PCR assay for blood mRNA Tg in the follow-up of patients with DTC.Design and Patients: An assay employing primers located in a region not affected by alternative splicing or single nucleotide polymorphisms was developed to study 104 DTC patients (13 of 104 with positive TgAb).Results: the assay is specific for thyroid tissue because we found mRNA Tg expression in normal thyroid tissue, but we did not find any mRNA Tg expression in any extrathyroidal tissues. Quantitative mRNA Tg levels were significantly different between patients free of disease (82 of 104) and those with metastases (22 of 104) (2.61 +/- 0.26 vs. 27.58 +/- 1.62 pg mRNA Tg/mu g RNA) (P < 0.0001). A cutoff point of 5.51 was able to discriminate between the two groups. in addition, the measurement of mRNA Tg was not affected by the presence of TgAb.Conclusion: This new mRNA Tg quantification is a reliable method that allowed us to differentiate patients free of disease from those with metastases, and it could represent an appropriate molecular marker for the follow-up of patients with DTC, especially those with positive TgAb. (J Clin Endocrinol Metab 95: 1726-1733, 2010)
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Research-Fellowship Grant
Número do financiamento FAPESP: 04/09934-7
Research-Fellowship Grant: 05/55842-0
Data de publicação 2010-04-01
Publicado em Journal of Clinical Endocrinology & Metabolism. Chevy Chase: Endocrine Soc, v. 95, n. 4, p. 1726-1733, 2010.
ISSN 0021-972X (Sherpa/Romeo, fator de impacto)
Publicador Endocrine Soc
Extensão 1726-1733
Fonte http://dx.doi.org/10.1210/jc.2009-1354
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000276402300030
Endereço permanente http://repositorio.unifesp.br/handle/11600/32393

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