Presence of mRNA of muscle nicotinic acetylcholine receptor subunits and an epsilon-subunit splice variant in the mouse brain

Presence of mRNA of muscle nicotinic acetylcholine receptor subunits and an epsilon-subunit splice variant in the mouse brain

Autor Ghedini, P. C. Autor UNIFESP Google Scholar
Honda, L. Autor UNIFESP Google Scholar
Avellar, M. C. W. Autor UNIFESP Google Scholar
Souccar, C. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Transcripts encoding for alpha 1, beta 1, delta, gamma and epsilon (and its splice variant epsilon(S)) subunits of the muscle-type nicotinic acetylcholine receptor (nAChR) were assessed using reverse transcription followed by polymerase chain reaction (RT-PCR) assays, with RNA extracted from the mouse skeletal muscle (diaphragm) and brain regions (cortex, hippocampus and cerebellum). the presence of alpha 1, beta 1, delta, gamma, epsilon and epsilon(S) transcripts was confirmed in the diaphragm muscle, used as positive control. mRNAs coding for muscle alpha 1, beta 1, delta, epsilon, epsilon(S), but not gamma subunits, were detected in adult mouse brain regions. An epsilon-subunit sequence variant, named epsilon(t), was also detected in all brain regions examined, but not in skeletal muscle. This new epsilon-subunit splice variant lacks a 115 bp cassette corresponding to exon 8 in the first intracellular transmembrane domain of the subunit, leading to a truncated protein. the data provide evidence for the presence of muscle-type nAChR subunits in the mouse central nervous system. (C) 2009 Elsevier Inc. All Fights reserved.
Palavra-chave Nicotinic acetylcholine receptor
RT-PCR
Splice variant
Skeletal muscle
Central nervous system
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2010-03-16
Publicado em Brain Research Bulletin. Oxford: Pergamon-Elsevier B.V., v. 81, n. 4-5, p. 453-457, 2010.
ISSN 0361-9230 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 453-457
Fonte http://dx.doi.org/10.1016/j.brainresbull.2009.12.006
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000274972800015
Endereço permanente http://repositorio.unifesp.br/handle/11600/32381

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