Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium

Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium

Autor Rossi, Alexandre Guilherme Zabeu Autor UNIFESP Google Scholar
Gomes, Regina Célia Teixeira Autor UNIFESP Google Scholar
Simões, Manuel de Jesus Autor UNIFESP Google Scholar
Simões, Ricardo dos Santos Autor UNIFESP Google Scholar
Oliveira, Patricia B. Autor UNIFESP Google Scholar
Soares, Jose Maria Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Objective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.)
Assunto Hyperprolactinemia
prolactin receptor
Idioma Inglês
Data 2010-03-15
Publicado em Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010.
ISSN 0015-0282 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 1643-1649
Fonte http://dx.doi.org/10.1016/j.fertnstert.2009.02.021
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000276038700034
URI http://repositorio.unifesp.br/handle/11600/32380

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