Cell Phenotype Evaluation at Various Sites After Skin Transplantation and FTY720 Plus Sirolimus Therapy

Cell Phenotype Evaluation at Various Sites After Skin Transplantation and FTY720 Plus Sirolimus Therapy

Autor Lopes, C. T. Autor UNIFESP Google Scholar
Rosin, F. C. P. Autor UNIFESP Google Scholar
Cordeiro, J. A. Google Scholar
Bueno, V. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Med Sch Sao Jose Rio Preto
Resumo Cell phenotype evaluation enables better understanding of the rejection process in experimental transplantation. We studied allograft survival and the mechanisms associated with rejection in a murine model of skin transplantation in the absence of immunosuppression or after FTY720 or sirolimus (SRL) administration for 21 days. Leukocyte phenotype was evaluated in the peripheral blood, spleen, axillary lymph nodes, thymus gland, and skin graft using flow cytometry at 5 days posttransplantation. Treatment with FTY720 plus SRL increased skin allograft survival in association with lymphopenia, reduced CD11b+ and CD3+CD4+ cell percentages in the graft, decreased CD3+CD4+, CD3+CD8+, and CD11b+ cell counts in lymphoid organs, and decreased CD4+CD8+ cell count in the thymus. These results suggest that increased allograft survival in animals treated with F1Y720 plus SRL is due to possible impairment of antigen presentation or recognition in the graft and secondary lymphoid organs, and decreased emigration of mature thymocytes to the periphery.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento FAPESP: 04/14727-0
FAPESP: 07/56901-5
Data de publicação 2010-03-01
Publicado em Transplantation Proceedings. New York: Elsevier B.V., v. 42, n. 2, p. 573-577, 2010.
ISSN 0041-1345 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 573-577
Fonte http://dx.doi.org/10.1016/j.transproceed.2010.01.027
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000276051400045
Endereço permanente http://repositorio.unifesp.br/handle/11600/32351

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