The intrinsic apoptotic signaling pathway in gastric adenocarcinomas of Brazilian patients: Immunoexpression of the Bcl-2 family (Bcl-2, Bcl-x, Bak, Bax, Bad) determined by tissue microarray analysis

The intrinsic apoptotic signaling pathway in gastric adenocarcinomas of Brazilian patients: Immunoexpression of the Bcl-2 family (Bcl-2, Bcl-x, Bak, Bax, Bad) determined by tissue microarray analysis

Author Barrezueta, Luis Fernando Mesias Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Lima, Flavio Oliveira Autor UNIFESP Google Scholar
Costa, Henrique de Oliveira Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Artigiani Neto, Ricardo Autor UNIFESP Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract This Study was undertaken to investigate the immunoexpression of Bcl-2 family proteins (Bcl-2, Bcl-x, Bax, Bak and Bad) and to evaluate the correlation between the immunoexpression of these proteins and that of cleaved caspase 3, Ki-67 and p53. A tissue microarray (TMA) paraffin block was constructed using gastric carcinoma tissue (test group) and adjacent normal gastric mucosa (control group) from 87 patients who had not previously undergone radiotherapy or chemotherapy. Sections from the TMA block (4 mu m) were subjected to immunohistochemistry for Bcl-2, Bcl-x, Bax, Bak, Bad, p53, Ki-67 and cleaved caspase-3. the slides were evaluated by the semi-quantitative method. and the scores obtained (intensity vs. percentage of staining) were correlated with one another and with the apoptotic index, cellular proliferation and data regarding patient survival. the studied proteins were present in the tumor tissue and in the normal (gastric mucosa, but at different intensities and with differences in the number of positive cells. There was all association between tumor size and p53 expression, and intestinal type adenocarcinoma was positively correlated with the expression of Bax, Bad and Ki-67. the immunoexpression of Bcl-x, Bak, Bad, p53 and Ki-67 showed statistically significant differences between the tumor tissue and the adjacent normal gastric mucosa. There was all association between the expression of Bax, Bak and Bad in the normal gastric mucosa. No correlation between patient survival and the expression of these proteins was observed. Overexpression of the Bcl-x protein in the adenocarcinomas and the difference ill Bcl-x expression between the test and the control group may be related to the anti-apoptotic effect of this protein. the reduced expression of Bak and Bad and the increased expression of p53 and Ki-67 in the adenocarcinomas demonstrate the imbalance between apoptosis and cellular proliferation, which results in uncontrolled tumor cell proliferation.
Keywords gastric cancer
apoptosis
family Bcl-2
immunohistochemistry
prognosis
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CAPES: 45779
FAPESP: 2004/09932-4
FAPESP: 06/54187-0
Date 2010-03-01
Published in Molecular Medicine Reports. Athens: Spandidos Publ Ltd, v. 3, n. 2, p. 261-267, 2010.
ISSN 1791-2997 (Sherpa/Romeo, impact factor)
Publisher Spandidos Publ Ltd
Extent 261-267
Origin http://www.spandidos-publications.com/mmr/3/2/261
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000274822200010
URI http://repositorio.unifesp.br/handle/11600/32309

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