Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells

Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells

Author Vieira, Monica A. M. Autor UNIFESP Google Scholar
Gomes, Tania A. T. Autor UNIFESP Google Scholar
Ferreira, Antonio J. P. Google Scholar
Knoebl, Terezinha Google Scholar
Servin, Alain L. Google Scholar
Lievin-Le Moal, Vanessa Google Scholar
Institution INSERM
Univ Paris 11
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract In rabbit ligated ileal loops, two atypical enteropathogenic Escherichia coli (aEPEC) strains, 3991-1 and 0421-1, intimately associated with the cell membrane, forming the characteristic EPEC attachment and effacement lesion of the brush border, induced a mucous hypersecretion, whereas typical EPEC (tEPEC) strain E2348/69 did not. Using cultured human mucin-secreting intestinal HT29-MTX cells, we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. the transcription of the MUC5AC and MUC4 genes was transiently upregulated after aEPEC infection. We provide evidence that the apically adhering aEPEC cells exploit the mucins' increased production since they grew in the presence of membrane-bound mucins, whereas tEPEC did not. the data described herein report a putative new virulence phenomenon in aEPEC.
Language English
Sponsor Institut National de la Sante et de la Recherche Medicale (Inserm)
Universite Paris-Sud 11
Ministere de la Recherche
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-03-01
Published in Infection and Immunity. Washington: Amer Soc Microbiology, v. 78, n. 3, p. 927-938, 2010.
ISSN 0019-9567 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 927-938
Origin http://dx.doi.org/10.1128/IAI.01115-09
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000274654200005
URI http://repositorio.unifesp.br/handle/11600/32296

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