Involvement of proteinase-activated receptors 1 and 2 in spreading and phagocytosis by murine adherent peritoneal cells: Modulation by the C-terminal of S100A9 protein

Show simple item record

dc.contributor.author Pagano, Rosana L.
dc.contributor.author Sampaio, Sandra C.
dc.contributor.author Juliano, Maria A. [UNIFESP]
dc.contributor.author Juliano, Luiz [UNIFESP]
dc.contributor.author Giorgi, Renata
dc.date.accessioned 2016-01-24T13:59:20Z
dc.date.available 2016-01-24T13:59:20Z
dc.date.issued 2010-02-25
dc.identifier http://dx.doi.org/10.1016/j.ejphar.2009.11.033
dc.identifier.citation European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 628, n. 1-3, p. 240-246, 2010.
dc.identifier.issn 0014-2999
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32276
dc.description.abstract Proteinase-activated receptors (PAR) are widely recognized for their modulatory properties in inflammatory and immune responses; however, their direct role on phagocyte effector functions remains unknown. S100A9, a protein secreted during inflammatory responses, deactivates activated peritoneal macrophages, and its C-terminal portion inhibits spreading and phagocytosis of adherent peritoneal cells. Herein, the effect of PAR1 and PAR2 agonists was investigated on spreading and phagocytosis by adherent peritoneal cells, as well as the ability of murine C-terminal of S100A9 peptide (mS100A9p) to modulate this effect. Adherent peritoneal cells obtained from mouse abdominal cavity were incubated with PAR1 and PAR2 agonists and spreading and phagocytosis of Candida albicans particles were evaluated. PAR1 agonists increased both the spreading and the phagocytic activity, but PAR2 agonists only increased the spreading index. mS100A9p reverted both the increased spreading and phagocytosis induced by PAR1 agonists, but no interference in the increased spreading induced by PAR2 agonists was noticed. the shorter homologue peptide to the C-terminal of mS100A9p, corresponding to the H(92)-E(97) region, also reverted the increased spreading and phagocytosis induced by PAR1 agonists. These findings show that proteinase-activated receptors have an important role for spreading and phagocytosis of adherent peritoneal cells, and that the pepticle corresponding to the C-terminal of S100A9 protein is a remarkable candidate for use as a novel compound to modulate PAR1 function. (C) 2009 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Fundacao Butantan
dc.format.extent 240-246
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof European Journal of Pharmacology
dc.rights Acesso restrito
dc.subject Proteinase-activated receptor en
dc.subject Adherent peritoneal cell en
dc.subject Phagocytosis en
dc.subject Spreading en
dc.subject S100A9 en
dc.subject (Mouse) en
dc.title Involvement of proteinase-activated receptors 1 and 2 in spreading and phagocytosis by murine adherent peritoneal cells: Modulation by the C-terminal of S100A9 protein en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Butantan Inst
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Butantan Inst, Lab Pathophysiol, BR-05503900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Inst Pharmacol, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Inst Pharmacol, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/j.ejphar.2009.11.033
dc.description.source Web of Science
dc.identifier.wos WOS:000274784500034



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account