Immobilization of biomolecules on nanostructured films for biosensing

Immobilization of biomolecules on nanostructured films for biosensing

Author Siqueira, Jose R. Google Scholar
Caseli, Luciano Autor UNIFESP Google Scholar
Crespilho, Frank N. Google Scholar
Zucolotto, Valtencir Google Scholar
Oliveira, Osvaldo N. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do ABC (UFABC)
Abstract This paper brings an overview of the use of nanostructured films in several types of biosensors, with emphasis on the advantageous control of molecular architecture which is typical of the layer-by-layer (LbL) and Langmuir-Blodgett films. Following introductory sections on film fabrication and detection methods, we concentrate on the immobilization of biomolecules on these nanostructured films used in units for biosensing. Important contributions in the literature in biosensors based on electrochemical and optical measurements are highlighted. Furthermore, a discussion is presented on how the concept of electronic tongues has been extended to biosensing, which resulted in increased sensitivity and selectivity. the integration of sensing units with micro-electronics is commented upon, especially in the context of using field-effect transistors (FETs) for biosensing. Examples of LbL and LB films containing proteins, lipids, metallic nanoparticles and carbon nanotubes, which are used for detecting a variety of analytes, will be provided. the prospects for clinical diagnosis with such biosensors are also assessed. Throughout the review, emphasis is placed on the importance of control of molecular architecture, particularly with synergistic combination of organic and inorganic materials. for example, nanostructured films containing capped gold nanoparticles or carbon nanotubes exhibited enhanced performance in biosensing. It is hoped that this survey may assist researchers in choosing materials, molecular architectures, and detection principles, which may be tailored for specific applications. (C) 2009 Elsevier B.V. All rights reserved.
Keywords Biosensors
Langmuir-Blodgett
Layer-by-layer films
Proteins
Detection principles
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
INEO
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2010-02-15
Published in Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, v. 25, n. 6, p. 1254-1263, 2010.
ISSN 0956-5663 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1254-1263
Origin http://dx.doi.org/10.1016/j.bios.2009.09.043
Access rights Closed access
Type Review
Web of Science ID WOS:000274868800001
URI http://repositorio.unifesp.br/handle/11600/32272

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