Two physically, functionally, and developmentally distinct peritoneal macrophage subsets

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dc.contributor.author Ghosn, Eliver Eid Bou
dc.contributor.author Cassado, Alexandra A.
dc.contributor.author Govoni, Gregory R.
dc.contributor.author Fukuhara, Takeshi
dc.contributor.author Yang, Yang
dc.contributor.author Monack, Denise M.
dc.contributor.author Bortoluci, Karina Ramalho [UNIFESP]
dc.contributor.author Almeida, Sandro R.
dc.contributor.author Herzenberg, Leonard
dc.contributor.author Herzenberg, Leonore A.
dc.date.accessioned 2016-01-24T13:59:19Z
dc.date.available 2016-01-24T13:59:19Z
dc.date.issued 2010-02-09
dc.identifier http://dx.doi.org/10.1073/pnas.0915000107
dc.identifier.citation Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 107, n. 6, p. 2568-2573, 2010.
dc.identifier.issn 0027-8424
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32262
dc.description.abstract The peritoneal cavity (PerC) is a unique compartment within which a variety of immune cells reside, and from which macrophages (Mempty set) are commonly drawn for functional studies. Here we define two Mempty set subsets that coexist in PerC in adult mice. One, provisionally called the large peritoneal Mempty set (LPM), contains approximately 90% of the PerC Mempty set in unstimulated animals but disappears rapidly from PerC following lipopolysaccharide (LPS) or thioglycolate stimulation. These cells express high levels of the canonical Mempty set surface markers, CD11b and F4/80. the second subset, referred to as small peritoneal Mempty set (SPM), expresses substantially lower levels of CD11b and F4/80 but expresses high levels of MHC-II, which is not expressed on LPM. SPM, which predominates in PerC after LPS or thioglycolate stimulation, does not derive from LPM. Instead, it derives from blood monocytes that rapidly enter the PerC after stimulation and differentiate to mature SPM within 2 to 4 d. Both subsets show clear phagocytic activity and both produce nitric oxide (NO) in response to LPS stimulation in vivo. However, their responses to LPS show key differences: in vitro, LPS stimulates LPM, but not SPM, to produce NO; in vivo, LPS stimulates both subsets to produce NO, albeit with different response patterns. These findings extend current models of Mempty set heterogeneity and shed new light on PerC Mempty set diversity, development, and function. Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC Mempty set. en
dc.description.sponsorship National Institutes of Health
dc.format.extent 2568-2573
dc.language.iso eng
dc.publisher Natl Acad Sciences
dc.relation.ispartof Proceedings of the National Academy of Sciences of the United States of America
dc.rights Acesso aberto
dc.subject CD11b en
dc.subject F4/80 en
dc.subject lipopolysaccharide en
dc.subject peritoneal cavity en
dc.subject thioglycolate en
dc.title Two physically, functionally, and developmentally distinct peritoneal macrophage subsets en
dc.type Artigo
dc.contributor.institution Stanford Univ
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA
dc.description.affiliation Univ São Paulo, Fac Pharmaceut Sci, Dept Clin Anal, BR-05588 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Biol Sci ICB IV, Dept Immunol, BR-05588 São Paulo, Brazil
dc.description.affiliation Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
dc.description.affiliation Universidade Federal de São Paulo, Dept Biol Sci, BR-09972 Diadema, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biol Sci, BR-09972 Diadema, SP, Brazil
dc.description.sponsorshipID National Institutes of Health: AI076434
dc.identifier.doi 10.1073/pnas.0915000107
dc.description.source Web of Science
dc.identifier.wos WOS:000274408100039



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