Two physically, functionally, and developmentally distinct peritoneal macrophage subsets

Two physically, functionally, and developmentally distinct peritoneal macrophage subsets

Author Ghosn, Eliver Eid Bou Google Scholar
Cassado, Alexandra A. Google Scholar
Govoni, Gregory R. Google Scholar
Fukuhara, Takeshi Google Scholar
Yang, Yang Google Scholar
Monack, Denise M. Google Scholar
Bortoluci, Karina Ramalho Autor UNIFESP Google Scholar
Almeida, Sandro R. Google Scholar
Herzenberg, Leonard Google Scholar
Herzenberg, Leonore A. Google Scholar
Institution Stanford Univ
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The peritoneal cavity (PerC) is a unique compartment within which a variety of immune cells reside, and from which macrophages (Mempty set) are commonly drawn for functional studies. Here we define two Mempty set subsets that coexist in PerC in adult mice. One, provisionally called the large peritoneal Mempty set (LPM), contains approximately 90% of the PerC Mempty set in unstimulated animals but disappears rapidly from PerC following lipopolysaccharide (LPS) or thioglycolate stimulation. These cells express high levels of the canonical Mempty set surface markers, CD11b and F4/80. the second subset, referred to as small peritoneal Mempty set (SPM), expresses substantially lower levels of CD11b and F4/80 but expresses high levels of MHC-II, which is not expressed on LPM. SPM, which predominates in PerC after LPS or thioglycolate stimulation, does not derive from LPM. Instead, it derives from blood monocytes that rapidly enter the PerC after stimulation and differentiate to mature SPM within 2 to 4 d. Both subsets show clear phagocytic activity and both produce nitric oxide (NO) in response to LPS stimulation in vivo. However, their responses to LPS show key differences: in vitro, LPS stimulates LPM, but not SPM, to produce NO; in vivo, LPS stimulates both subsets to produce NO, albeit with different response patterns. These findings extend current models of Mempty set heterogeneity and shed new light on PerC Mempty set diversity, development, and function. Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC Mempty set.
Keywords CD11b
peritoneal cavity
Language English
Sponsor National Institutes of Health
Grant number National Institutes of Health: AI076434
Date 2010-02-09
Published in Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 107, n. 6, p. 2568-2573, 2010.
ISSN 0027-8424 (Sherpa/Romeo, impact factor)
Publisher Natl Acad Sciences
Extent 2568-2573
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000274408100039

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