Differences in the expression of glycosaminoglycans in human fibroblasts derived from gingival overgrowths is related to TGF-beta up-regulation

Differences in the expression of glycosaminoglycans in human fibroblasts derived from gingival overgrowths is related to TGF-beta up-regulation

Author Dreyfuss, Juliana L. Autor UNIFESP Google Scholar
Veiga, Silv S. Google Scholar
Coulson-Thomas, Vivien Jane Autor UNIFESP Google Scholar
Santos, Isabel A. Autor UNIFESP Google Scholar
Toma, Leny Autor UNIFESP Google Scholar
Coletta, Ricardo D. Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Parana
Universidade Estadual de Campinas (UNICAMP)
Abstract Glycosaminoglycans (GAGs) play important roles in cell behavior and have the ability to bind and modulate cytokines. Using primary cultured fibroblasts from hereditary gingival fibromatosis (HGF), normal gingiva (NG), and NG treated with cyclosporin-A (NGc) we show changes in the expression and structural characteristics of GAGs as well as in the expression of enzymes involved in their biosynthesis and degradation. in addition, we show the over-expression of TGF-beta 1 and TGF-beta type 11 receptor in HGF and NGc. There is an increase in the GAGs retained in the cellular fraction, and the fine structure of galactosaminoglycans show a decrease in alpha-L-iduronic acid content in HGF and NGc. Elevated extracellular levels of low molecular weight hyaluronan (HA) are found in HGF due to increase in the expression of HA synthase 3 and hyaluronidases 1 and 2. the results bring new insights to the accumulation of extracellular matrix related to TGF-beta over-expression.
Keywords Sulfated glycosaminoglycans
hyaluronan
growth factors
hyaluronan synthases
hyaluronidases
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Jairo Ramos postdoctoral fellowship
FAP UNIFESP (Fundacao de Apoio a Pesquisa)
Date 2010-02-01
Published in Growth Factors. London: Informa Healthcare, v. 28, n. 1, p. 24-33, 2010.
ISSN 0897-7194 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 24-33
Origin http://dx.doi.org/10.3109/08977190903321819
Access rights Closed access
Type Article
Web of Science ID WOS:000275121800003
URI http://repositorio.unifesp.br/handle/11600/32258

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