Association Study of Vascular Endothelial Growth Factor and Polymorphisms of its Gene with Ectopic Pregnancy

Association Study of Vascular Endothelial Growth Factor and Polymorphisms of its Gene with Ectopic Pregnancy

Author Elito, Julio Autor UNIFESP Google Scholar
Daher, Silvia Autor UNIFESP Google Scholar
Fernandes da Silva, Marcelo Octavio Autor UNIFESP Google Scholar
Higashi Marconi, Natali Maiumi Autor UNIFESP Google Scholar
Tezotto Pendeloski, Karen Priscilla Autor UNIFESP Google Scholar
Moron, Antonio Fernandes Autor UNIFESP Google Scholar
Camano, Luiz Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract ProblemIn ectopic pregnancy, increased levels of vascular endothelial growth factor are present. the aims of this study were to determine the association between -634C/G, -460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.Method of studyThis is a case-control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post-menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. for 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (-634C/G, -460T/C, and +936C/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.ResultsThe -634C/G, -460T/C, and +936C/T VEGF polymorphisms were not associated with ectopic pregnancy (P = 0.170, P = 0.285, and P = 0.700, respectively). the serum levels of VEGF were not associated with the genotype of -634C/G, -460T/C, and +936C/T VEGF polymorphisms (P = 0.702; P = 0.347, and P = 0.256, respectively).ConclusionThere was no association between ectopic pregnancy and -634C/G, -460T/C, and +936C/T VEGF polymorphisms. There was no correlation between VEGF genotype and the expression of VEGF in blood samples.
Keywords Ectopic pregnancy
polymorphism
VEGF
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-02-01
Published in American Journal of Reproductive Immunology. Malden: Wiley-Blackwell, v. 63, n. 2, p. 120-125, 2010.
ISSN 1046-7408 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 120-125
Origin http://dx.doi.org/10.1111/j.1600-0897.2009.00769.x
Access rights Closed access
Type Article
Web of Science ID WOS:000273449400004
URI http://repositorio.unifesp.br/handle/11600/32253

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