Determination of polyamines in organic extracts from roots of Canavalia ensiformis by capillary electrophoresis

Determination of polyamines in organic extracts from roots of Canavalia ensiformis by capillary electrophoresis

Author Santos, S. Google Scholar
Moraes, M. L. L. Google Scholar
Rezende, M. O. O. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract A rapid, selective and specific capillary zone electrophoresis method to determine polyamines in organic extracts from roots of Canavalia ensiformis (Jack Beans) was developed using ultra violet (UV) detection. Canavalia ensiformis is relatively free from diseases and it is used as reference in allelopathy studies. Polyamines are widely distributed in plant and it could be involved in plant pathogen interactions. Optimal separation was achieved using 15 mmol.L-1formic acid (pH 3.0) + 4 mmol.L-1 imidazole as a background electrolyte. It was possible to identify and quantify the polyamines on herbal samples in the presence of other phytochemical substances and analyze them quickly (up to 6 min). the applicability of this method was evaluated in crude organic extracts from roots of Canavalia ensiformis.
Keywords Canavalia ensiformis
polyamines
capillary electrophoresis
allelopathy
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2010-01-01
Published in Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes. Philadelphia: Taylor & Francis Inc, v. 45, n. 4, p. 325-329, 2010.
ISSN 0360-1234 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 325-329
Origin http://dx.doi.org/10.1080/03601231003704481
Access rights Closed access
Type Article
Web of Science ID WOS:000277586700009
URI http://repositorio.unifesp.br/handle/11600/32125

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