A novel expression profile of the Loxosceles intermedia spider venomous gland revealed by transcriptome analysis

A novel expression profile of the Loxosceles intermedia spider venomous gland revealed by transcriptome analysis

Author Gremski, Luiza Helena Autor UNIFESP Google Scholar
Silveira, Rafael Bertoni da Google Scholar
Chaim, Olga Meiri Autor UNIFESP Google Scholar
Probst, Christian Macagnan Google Scholar
Ferrer, Valeria Pereira Google Scholar
Nowatzki, Jenifer Google Scholar
Weinschutz, Hellen Chris Google Scholar
Madeira, Humberto Maciel Google Scholar
Gremski, Waldemiro Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Senff-Ribeiro, Andrea Google Scholar
Veiga, Silvio Sanches Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Ponta Grossa
Univ Fed Parana
Fiocruz MS
Catholic Univ Parana
Abstract Spiders of the Loxosceles genus are cosmopolitan, and their venom components possess remarkable biological properties associated with their ability to act upon different molecules and receptors. Accidents with Loxosceles intermedia specimens are recognized as a public health problem in the south of Brazil. To describe the transcriptional profile of the L. intermedia venom gland, we generated a wide cDNA library, and its transcripts were functionally and structurally analyzed. After initial analyses, 1843 expressed sequence tags (ESTs) produced readable sequences that were grouped into 538 clusters, 281 of which were singletons. 985 reads (53% of total ESTs) matched to known proteins. Similarity searches showed that toxin-encoding transcripts account for 43% of the total library and comprise a great number of ESTs. the most frequent toxins were from the LiTx family, which are known for their insecticidal activity. Both phospholipase D and astacin-like metalloproteases toxins account for approximately 9% of total transcripts. Toxins components such as serine proteases, hyaluronidases and venom allergens were also found but with minor representation. Almost 10% of the ESTs encode for proteins involved in cellular processes. These data provide an important overview of the L. intermedia venom gland expression scenario and revealed significant differences from profiles of other spiders from the Loxosceles genus. Furthermore, our results also confirm that this venom constitutes an amazing source of novel compounds with potential agrochemical, industrial and pharmacological applications.
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao Araucaria
Secretaria de Estado de Ciencia
Tecnologia e Ensino Superior do Parana
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-01-01
Published in Molecular Biosystems. Cambridge: Royal Soc Chemistry, v. 6, n. 12, p. 2403-2416, 2010.
ISSN 1742-206X (Sherpa/Romeo, impact factor)
Publisher Royal Soc Chemistry
Extent 2403-2416
Origin http://dx.doi.org/10.1039/c004118a
Access rights Closed access
Type Article
Web of Science ID WOS:000283937800008
URI http://repositorio.unifesp.br/handle/11600/32120

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