The interaction of an antiparasitic peptide active against African Sleeping Sickness with cell membrane models

The interaction of an antiparasitic peptide active against African Sleeping Sickness with cell membrane models

Autor Pascholati, Caue P. Google Scholar
Lopera, Esteban Parra Google Scholar
Pavinatto, Felippe J. Google Scholar
Caseli, Luciano Autor UNIFESP Google Scholar
Nobre, Thatyane M. Google Scholar
Zaniquelli, Maria E. D. Google Scholar
Viitala, Tapani Google Scholar
D'Silva, Claudius Google Scholar
Oliveira, Osvaldo N. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Biolin Sci Oy
Manchester Metropolitan Univ
Resumo Zwitterionic peptides with trypanocidal activity are promising lead compounds for the treatment of African Sleeping Sickness, and have motivated research into the design of compounds capable of disrupting the protozoan membrane. in this study, we use the Langmuir monolayer technique to investigate the surface properties of an antiparasitic peptide, namely S-(2,4-dinitrophenyl)glutathione di-2-propyl ester, and its interaction with a model membrane comprising a phospholipid monolayer. the drug formed stable Langmuir monolayers. whose main feature was a phase transition accompanied by a negative surface elasticity. This was attributed to aggregation upon compression due to intermolecular bond associations of the molecules, inferred from surface pressure and surface potential isotherms. Brewster angle microscopy (BAM) images, infrared spectroscopy and dynamic elasticity measurements. When co-spread with dipalmitoyl phosphatidyl choline (DPPC). the drug affected both the surface pressure and the monolayer morphology, even at high surface pressures and with low amounts of the drug. the results were interpreted by assuming a repulsive, cooperative interaction between the drug and DPPC molecules. Such repulsive interaction and the large changes in fluidity arising from drug aggregation may be related to the disruption of the membrane, which is key for the parasite killing property. (C) 2009 Elsevier B.V. All rights reserved.
Assunto Langmuir monolayer
Langmuir-Blodgett film
African Sleeping Sickness
Drug
Peptide
Air-water interface
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data 2009-12-01
Publicado em Colloids and Surfaces B-biointerfaces. Amsterdam: Elsevier B.V., v. 74, n. 2, p. 504-510, 2009.
ISSN 0927-7765 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 504-510
Fonte http://dx.doi.org/10.1016/j.colsurfb.2009.08.018
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000272125100016
URI http://repositorio.unifesp.br/handle/11600/31988

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