HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles

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dc.contributor.author Mora, Azucena [UNIFESP]
dc.contributor.author Blanco, Miguel
dc.contributor.author Yamamoto, Denise [UNIFESP]
dc.contributor.author Dahbi, Ghizlane
dc.contributor.author Blanco, Jesus E.
dc.contributor.author Lopez, Cecilia
dc.contributor.author Alonso, Maria P.
dc.contributor.author Vieira, Monica A. M. [UNIFESP]
dc.contributor.author Hernandes, Rodrigo T. [UNIFESP]
dc.contributor.author Abe, Cecilia Mari [UNIFESP]
dc.contributor.author Piazza, Roxane M. F.
dc.contributor.author Lacher, David W.
dc.contributor.author Elias Junior, Waldir Pereira [UNIFESP]
dc.contributor.author Gomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.author Blanco, Jorge
dc.date.accessioned 2016-01-24T13:58:56Z
dc.date.available 2016-01-24T13:58:56Z
dc.date.issued 2009-12-01
dc.identifier http://dx.doi.org/10.2436/20.1501.01.104
dc.identifier.citation International Microbiology. Barcelona: Viguera Editores, S L, v. 12, n. 4, p. 243-251, 2009.
dc.identifier.issn 1139-6709
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31969
dc.description.abstract A collection of 69 eae-positive strains expressing 29 different intimin types and eight fir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BFP) expression. All of the nine typical enteropathogenic Escherichia coli (tEPEC) studied harbored the enteropathogenic E. coli adherence factor (EAF) plasmid, as shown by PCR and/or EAF probe results. in addition, they were positive for bfpA, as shown by PCR, and BFP expression, as confirmed by immunofluorescence (IFL) and/or immunoblotting (IBL) assays. Localized adherence (LA) was exclusively displayed by those nine tEPEC, while localized-adherence-like (LAL) was the most frequent pattern among atypical EPEC (aEPEC) and Shiga-toxin-producing E. coli (STEC). All LA and LAL strains were able to cause attaching and effacing (AE) lesions, as established by means of the FAS test. There was a significant association between the presence of tir allele alpha 1 and bfpA-positive strains, and consequently, with the LA pattern. However, intimin type or bfpA was not associated with the adherence pattern displayed in HeLa cells. Among the eight bfpA alleles detected, a new type (beta 10; accession number FN391178) was identified in a strain of serotype O157:H45, and a truncated variant (beta 3.2-t; accession number FN 39118 1) in four strains belonging to different pathotypes. [Int Microbiol 2009; 12(4):243-251] en
dc.description.sponsorship European Commission
dc.description.sponsorship Ministerio de Sanidad y Consumo de Espana
dc.description.sponsorship Ministerio de Educacion y Ciencia de Espana
dc.description.sponsorship Xunta de Galicia
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 243-251
dc.language.iso eng
dc.publisher Viguera Editores, S L
dc.relation.ispartof International Microbiology
dc.rights Acesso aberto
dc.subject enteropathogenic E. coli en
dc.subject Shiga-toxin-producing E. coli en
dc.subject HeLa-cell adherence en
dc.subject intimin en
dc.title HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles en
dc.type Artigo
dc.contributor.institution Univ Santiago de Compostela
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Butantan Inst
dc.contributor.institution Calde Xeral Hosp
dc.contributor.institution US FDA
dc.description.affiliation Univ Santiago de Compostela, Fac Vet Sci, Dept Microbiol & Parasitol, Lugo 27002, Spain
dc.description.affiliation Universidade Federal de São Paulo, São Paulo Sch Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliation Butantan Inst, Bacteriol Lab, São Paulo, Brazil
dc.description.affiliation Calde Xeral Hosp, Microbiol Unit, Lugo, Spain
dc.description.affiliation US FDA, Div Mol Biol, Ctr Food Safety & Appl Nutr, Laurel, MD USA
dc.description.affiliationUnifesp Universidade Federal de São Paulo, São Paulo Sch Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sponsorshipID European Commission: FAIR6-CT-4093
dc.description.sponsorshipID European Commission: FOOD-CT-2006-36256
dc.description.sponsorshipID Ministerio de Sanidad y Consumo de Espana: FIS G03-025-COLIRED-O157
dc.description.sponsorshipID Ministerio de Sanidad y Consumo de Espana: PI052023
dc.description.sponsorshipID Ministerio de Sanidad y Consumo de Espana: PI051481
dc.description.sponsorshipID Ministerio de Sanidad y Consumo de Espana: REIPI RD06/0008/1018-1016
dc.description.sponsorshipID Ministerio de Educacion y Ciencia de Espana: AGL-2008-02129
dc.description.sponsorshipID Xunta de Galicia: PGIDIT 05BTF26101P
dc.description.sponsorshipID Xunta de Galicia: PGIDIT065TAL26101P
dc.description.sponsorshipID Xunta de Galicia: 07MRU036261PR
dc.description.sponsorshipID Xunta de Galicia: 08TAL0172 61PR
dc.description.sponsorshipID Xunta de Galicia: 2007/000044-0
dc.description.sponsorshipID FAPESP: 08/53812-4
dc.identifier.file WOS000274010800006.pdf
dc.identifier.doi 10.2436/20.1501.01.104
dc.description.source Web of Science
dc.identifier.wos WOS:000274010800006



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