HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles

HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles

Author Mora, Azucena Autor UNIFESP Google Scholar
Blanco, Miguel Google Scholar
Yamamoto, Denise Autor UNIFESP Google Scholar
Dahbi, Ghizlane Google Scholar
Blanco, Jesus E. Google Scholar
Lopez, Cecilia Google Scholar
Alonso, Maria P. Google Scholar
Vieira, Monica A. M. Autor UNIFESP Google Scholar
Hernandes, Rodrigo T. Autor UNIFESP Google Scholar
Abe, Cecilia Mari Autor UNIFESP Google Scholar
Piazza, Roxane M. F. Google Scholar
Lacher, David W. Google Scholar
Elias Junior, Waldir Pereira Autor UNIFESP Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Blanco, Jorge Google Scholar
Institution Univ Santiago de Compostela
Universidade Federal de São Paulo (UNIFESP)
Butantan Inst
Calde Xeral Hosp
US FDA
Abstract A collection of 69 eae-positive strains expressing 29 different intimin types and eight fir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BFP) expression. All of the nine typical enteropathogenic Escherichia coli (tEPEC) studied harbored the enteropathogenic E. coli adherence factor (EAF) plasmid, as shown by PCR and/or EAF probe results. in addition, they were positive for bfpA, as shown by PCR, and BFP expression, as confirmed by immunofluorescence (IFL) and/or immunoblotting (IBL) assays. Localized adherence (LA) was exclusively displayed by those nine tEPEC, while localized-adherence-like (LAL) was the most frequent pattern among atypical EPEC (aEPEC) and Shiga-toxin-producing E. coli (STEC). All LA and LAL strains were able to cause attaching and effacing (AE) lesions, as established by means of the FAS test. There was a significant association between the presence of tir allele alpha 1 and bfpA-positive strains, and consequently, with the LA pattern. However, intimin type or bfpA was not associated with the adherence pattern displayed in HeLa cells. Among the eight bfpA alleles detected, a new type (beta 10; accession number FN391178) was identified in a strain of serotype O157:H45, and a truncated variant (beta 3.2-t; accession number FN 39118 1) in four strains belonging to different pathotypes. [Int Microbiol 2009; 12(4):243-251]
Keywords enteropathogenic E. coli
Shiga-toxin-producing E. coli
HeLa-cell adherence
intimin
Language English
Sponsor European Commission
Ministerio de Sanidad y Consumo de Espana
Ministerio de Educacion y Ciencia de Espana
Xunta de Galicia
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number European Commission: FAIR6-CT-4093
European Commission: FOOD-CT-2006-36256
Ministerio de Sanidad y Consumo de Espana: FIS G03-025-COLIRED-O157
Ministerio de Sanidad y Consumo de Espana: PI052023
Ministerio de Sanidad y Consumo de Espana: PI051481
Ministerio de Sanidad y Consumo de Espana: REIPI RD06/0008/1018-1016
Ministerio de Educacion y Ciencia de Espana: AGL-2008-02129
Xunta de Galicia: PGIDIT 05BTF26101P
Xunta de Galicia: PGIDIT065TAL26101P
Xunta de Galicia: 07MRU036261PR
Xunta de Galicia: 08TAL0172 61PR
Xunta de Galicia: 2007/000044-0
FAPESP: 08/53812-4
Date 2009-12-01
Published in International Microbiology. Barcelona: Viguera Editores, S L, v. 12, n. 4, p. 243-251, 2009.
ISSN 1139-6709 (Sherpa/Romeo, impact factor)
Publisher Viguera Editores, S L
Extent 243-251
Origin http://dx.doi.org/10.2436/20.1501.01.104
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000274010800006
URI http://repositorio.unifesp.br/handle/11600/31969

Show full item record




File

Name: WOS000274010800006.pdf
Size: 71.06Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account