Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: Expression profile of genes in the nitric oxide pathway of penile tissue of mice

Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: Expression profile of genes in the nitric oxide pathway of penile tissue of mice

Author Villanova, Fabiola E. Google Scholar
Andrade, Enrico Google Scholar
Leal, Elcio Autor UNIFESP Google Scholar
Andrade, Priscila M. Google Scholar
Borra, Ricardo C. Google Scholar
Troncone, Lanfranco R. P. Google Scholar
Magalhaes, Leandro Google Scholar
Leite, Katia R. M. Google Scholar
Paranhos, Mario Google Scholar
Claro, Joaquim Google Scholar
Srougi, Miguel Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Ibirapuera
Butantan Inst
Abstract The peptides Tx2-5 and Tx2-6, isolated from the whole venom of armed-spider Phoneutria nigniventer venom, are directly linked with the induction of persistent and painful erection in the penis of mammals. the erection induced by Tx2-6 has been associated with the activation of nitric oxide synthases. There is a scarcity of studies focusing on the outcome of Tx2-6 at the molecular level, by this reason we evaluated the gene profile activity of this toxin at the nitric oxide (NO) pathway. After microarray analyses on cavernous tissue of mice inoculated with Tx2-6 we found that only 10.4% (10/96) of these genes were differentially expressed, showing a limited effect of the toxin on the NO pathway. We found the genes sparc, ednrb, junb, cdkn1a, bcl2, ccl5, abcc1 over-expressed and the genes sod1, s100a10 and fth1 under-expressed after inoculation of Tx2-6. the differential expressions of sparc and ednrb genes were further confirmed using real-time PCR. Interestingly, ednrb activates the L-arginine/NO/cGMP pathway that is involved in the relaxation of the cavernous body. Therefore the priapism induced by Tx2-6 is a consequence of a highly specific interference of this neurotoxin with the NO pathway. (C) 2009 Elsevier B.V. All rights reserved.
Keywords Phoneutria nigriventer
Tx2-6
Gene expression
Spider toxin
Nitric oxide
Penile erection
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 06/57922-3
FAPESP: 06157923-0
FAPESP: 04/10372-3
FAPESP: 07/52841-8
Date 2009-11-01
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 54, n. 6, p. 793-801, 2009.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 793-801
Origin http://dx.doi.org/10.1016/j.toxicon.2009.06.006
Access rights Closed access
Type Article
Web of Science ID WOS:000270626900011
URI http://repositorio.unifesp.br/handle/11600/31928

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