Case Report: Successful Sporozoite Challenge Model in Human Volunteers with Plasmodium vivax Strain Derived from Human Donors

Case Report: Successful Sporozoite Challenge Model in Human Volunteers with Plasmodium vivax Strain Derived from Human Donors

Author Herrera, Socrates Google Scholar
Fernandez, Olga Google Scholar
Manzano, Maria R. Google Scholar
Murrain, Bermans Google Scholar
Vergara, Juana Google Scholar
Blanco, Pedro Google Scholar
Palacios, Ricardo Autor UNIFESP Google Scholar
Velez, Juan Diego Google Scholar
Epstein, Judith E. Google Scholar
Chen-Mok, Mario Google Scholar
Reed, Zarifah Hussain Google Scholar
Arevalo-Herrera, Myriam Google Scholar
Institution Ctr Int Vacunas
Univ Valle
Univ Nacl Colombia
Universidade Federal de São Paulo (UNIFESP)
Fdn Clin Valle Lili
USN
Family Hlth Int
Reg Emerging Dis Intervent REDI Ctr
WHO
Abstract Successful establishment of a Plasmodium vivax sporozoite challenge model in humans is described. Eighteen healthy adult, malaria-naive volunteers were randomly allocated to Groups A-C and exposed to 3 +/- 1, 6 +/- 1, and 9 +/- 1 bites of Anopheles albimanus mosquitoes infected with P vivax, respectively. Seventeen volunteers developed signs and symptoms consistent with malaria, and geometric mean prepatent periods of 11.1 days (9.3-11) for Group A; 10.8 days (9.8-11.9) for Group B; and 10.6 days (8.7-12.4) for Group C, with no statistically significant difference among groups (Kruskal-Wallis, P = 0.70). One volunteer exposed to eight mosquito bites did not develop a parasitemia. No differences in parasite density were observed and all individuals successfully recovered after anti-malarial treatment. None of the volunteers developed parasite relapses within an 18-month follow-up. in conclusion, malaria-naive volunteers can be safely and reproducibly infected with bites of 2-10 An. albimanus mosquitoes carrying P vivax. sporozoites. This challenge method is suitable for vaccine and anti-malarial drug testing.
Language English
Sponsor World Health Organization Initiative for Vaccine Research
National Institute of Allergy and Infectious Diseases NIAID
Colombian National Research Council
COLCIENCIAS
Malaria Vaccine and Drug Development Center Foundation
Grant number World Health Organization Initiative for Vaccine Research: LA35735G
National Institute of Allergy and Infectious Diseases NIAID: AI49486-05/TMRC
Date 2009-11-01
Published in American Journal of Tropical Medicine and Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 81, n. 5, p. 740-746, 2009.
ISSN 0002-9637 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Trop Med & Hygiene
Extent 740-746
Origin http://dx.doi.org/10.4269/ajtmh.2009.09-0194
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000271956500003
URI http://repositorio.unifesp.br/handle/11600/31909

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