Biomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue

Biomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue

Author Martins, Renato A. Autor UNIFESP Google Scholar
Silva Gomes, Guilherme A. da Autor UNIFESP Google Scholar
Aguiar, Odair Autor UNIFESP Google Scholar
Ribeiro, Daniel A. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Owing to the influence of geno- and cytotoxicity on chemical carcinogenesis, studies have demonstrated that petroleum derivatives are able to induce genetic damage and cellular death with conflicting results so far. the aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from gas petrol attendants using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 23 gas petrol attendants and 23 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. the results pointed out significant statistical differences (p<0.05) of micronucleated oral mucosa cells from gas petrol attendants for both oral sites evaluated. in the same way, petroleum derivate exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis, being the most pronunciated effects as those found in the lateral border of the tongue. No interaction was observed between smoking and petroleum exposure. in summary, these data indicate that gas petrol attendants comprise a high risk group for DNA damage and cellular death. It seems that the lateral border of the tongue is a more sensitive site to geno- and cytotoxic insult induced by petroleum derivates. (C) 2009 Elsevier B.V. All rights reserved.
Keywords Buccal mucosa cells
Tongue cells
Petroleum derivates
Miconucleus test
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2007/00345-7
FAPESP: 2007/01228-4
Date 2009-10-01
Published in Environment International. Oxford: Pergamon-Elsevier B.V., v. 35, n. 7, p. 1062-1065, 2009.
ISSN 0160-4120 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1062-1065
Origin http://dx.doi.org/10.1016/j.envint.2009.06.001
Access rights Closed access
Type Article
Web of Science ID WOS:000270312600010
URI http://repositorio.unifesp.br/handle/11600/31857

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