The Flagella of an Atypical Enteropathogenic Escherichia coli Strain Are Required for Efficient Interaction with and Stimulation of Interleukin-8 Production by Enterocytes in Vitro

The Flagella of an Atypical Enteropathogenic Escherichia coli Strain Are Required for Efficient Interaction with and Stimulation of Interleukin-8 Production by Enterocytes in Vitro

Author Sampaio, Suely C. F. Google Scholar
Gomes, Tânia Aparecida Tardelli Autor UNIFESP Google Scholar
Pichon, Christophe Google Scholar
du Merle, Laurence Google Scholar
Guadagnini, Stephanie Google Scholar
Abe, Cecilia Mari Autor UNIFESP Google Scholar
Sampaio, Jorge L. M. Google Scholar
Le Bouguenec, Chantal Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Pasteur
Inst Butantan
Inst Fleury Ensino & Pesquisa
Abstract The ability of some typical enteropathogenic Escherichia coli (EPEC) strains to adhere to, invade, and increase interleukin-8 (IL-8) production in intestinal epithelial cells in vitro has been demonstrated. However, few studies regarding these aspects have been performed with atypical EPEC (aEPEC) strains, which are emerging enteropathogens in Brazil. in this study, we evaluated a selected aEPEC strain (1711-4) of serotype O51:H40, the most prevalent aEPEC serotype in Brazil, in regard to its ability to adhere to and invade Caco-2 and T84 cells and to elicit IL-8 production in Caco-2 cells. the role of flagella in aEPEC 1711-4 adhesion, invasion, and IL-8 production was investigated by performing the same experiments with an isogenic aEPEC mutant unable to produce flagellin (FliC), the flagellum protein subunit. We demonstrated that this mutant (fliC mutant) had a marked decrease in the ability to adhere to T84 cells and invade both T84 and Caco-2 cells in gentamicin protection assays and by transmission electron microscopy. in addition, the aEPEC 1711-4 fliC mutant had a reduced ability to stimulate IL-8 production by Caco-2 cells in early (3-h) but not in late (24-h) infections. Our findings demonstrate that flagella of aEPEC 1711-4 are required for efficient adhesion, invasion, and early but not late IL-8 production in intestinal epithelial cells in vitro.
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Colegio Doutoral Franco Brasileiro
Institut Pasteur
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Programa de Apoio a Nucleos de Excelencia
PRONEX
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number Institut Pasteur: PTR165
FAPESP: 05/59128-0
Date 2009-10-01
Published in Infection and Immunity. Washington: Amer Soc Microbiology, v. 77, n. 10, p. 4406-4413, 2009.
ISSN 0019-9567 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 4406-4413
Origin http://dx.doi.org/10.1128/IAI.00177-09
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000269952800024
URI http://repositorio.unifesp.br/handle/11600/31841

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