Strain-specific protective immunity following vaccination against experimental Trypanosoma cruzi infection

Strain-specific protective immunity following vaccination against experimental Trypanosoma cruzi infection

Author Haolla, Filipe A. Autor UNIFESP Google Scholar
Claser, Carla Autor UNIFESP Google Scholar
Alencar, Bruna C. G. de Autor UNIFESP Google Scholar
Tzelepis, Fanny Autor UNIFESP Google Scholar
Vasconcelos, Jose Ronnie de Autor UNIFESP Google Scholar
Oliveira, Gabriel de Google Scholar
Silverio, Jaline C. Google Scholar
Machado, Alexandre V. Google Scholar
Lannes-Vieira, Joseli Google Scholar
Bruna-Romero, Oscar Google Scholar
Gazzinelli, Ricardo T. Google Scholar
Santos, Ricardo Ribeiro dos Google Scholar
Soares, Milena B. P. Google Scholar
Rodrigues, Mauricio M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fiocruz MS
Universidade Federal de Minas Gerais (UFMG)
Univ Massachusetts
Hosp Sao Rafael
Abstract Immunisation with Amastigote Surface Protein 2 (asp-2) and trans-sialidase (ts) genes induces protective immunity in highly susceptible A/Sn mice, against infection with parasites of the Y strain of Trypanosoma cruzi. Based on immunological and biological strain variations in T cruzi parasites, our goal was to validate our vaccination results using different parasite strains. Due to the importance of the CD8(+) T cells in protective immunity, we initially determined which strains expressed the immunodominant H-2K(k)-restricted epitope TEWETGQI. We tested eight strains, four of which elicited immune responses to this epitope (Y, G, Colombian and Colombia). We selected the Colombian and Colombia strains for our studies. A/Sn mice were immunised with different regimens using both T. cruzi genes (asp-2 and ts) simultaneously and subsequently challenged with blood trypomastigotes. Immune responses before the challenge were confirmed by the presence of specific antibodies and peptide-specific T cells. Genetic vaccination did not confer protective immunity against acute infection with a lethal dose of the Colombian strain. in contrast, we observed a drastic reduction in parasitemia and a significant increase in survival, following challenge with an otherwise lethal dose of the Colombia strain. in many surviving animals with late-stage chronic infection, we observed alterations in the heart's electrical conductivity, compared to naive mice. in summary, we concluded that immunity against T cruzi antigens, similar to viruses and bacteria, may be strain-specific and have a negative impact on vaccine development. (c) 2009 Elsevier B.V. All rights reserved.
Keywords Trypanosoma cruzi
DNA vaccine
Adenovirus vaccine
CD8
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Grant number FAPESP: 2006/1983-4
CNPq: 420067/2005-1
FAPEMIG: EDT 24.000
Date 2009-09-18
Published in Vaccine. Oxford: Elsevier B.V., v. 27, n. 41, p. 5644-5653, 2009.
ISSN 0264-410X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 5644-5653
Origin http://dx.doi.org/10.1016/j.vaccine.2009.07.013
Access rights Closed access
Type Article
Web of Science ID WOS:000270070400015
URI http://repositorio.unifesp.br/handle/11600/31818

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