Proteomic analysis of total cellular proteins of human neutrophils

Show simple item record

dc.contributor.author Tomazella, Gisele G. [UNIFESP]
dc.contributor.author Silva, Idalete da
dc.contributor.author Laure, Helen J.
dc.contributor.author Rosa, Jose C.
dc.contributor.author Chammas, Roger
dc.contributor.author Wiker, Harald G.
dc.contributor.author Souza, Gustavo A. de
dc.contributor.author Greene, Lewis J. [UNIFESP]
dc.date.accessioned 2016-01-24T13:58:38Z
dc.date.available 2016-01-24T13:58:38Z
dc.date.issued 2009-08-31
dc.identifier http://dx.doi.org/10.1186/1477-5956-7-32
dc.identifier.citation Proteome Science. London: Biomed Central Ltd, v. 7, 9 p., 2009.
dc.identifier.issn 1477-5956
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31749
dc.description.abstract Background: Neutrophils are the most abundant leukocytes in peripheral blood and represent one of the most important elements of innate immunity. Recent subcellular proteomic studies have focused on the identification of human neutrophil proteins in various subcellular membrane and granular fractions. Although there are relatively few studies dealing with the analysis of the total extract of human neutrophils, many biological problems such as the role of chemokines, adhesion molecules, and other activating inputs involved in neutrophil responses and signaling can be approached on the basis of the identification of the total cellular proteins.Results: Using gel-LC-MS/MS, 251 total cellular proteins were identified from resting human neutrophils. This is more than ten times the number of proteins identified by an initial proteome analysis of human neutrophils and almost five times the number of proteins identified by the first 2-DE map of extracts of rat polymorphonuclear leukocytes. Most of the proteins identified in the present study are well-known, but some of them, such as neutrophil-secreted proteins and centaurin beta-1, a cytoplasmic protein involved in the regulation of NF-kappa B activity, are described here for the first-time.Conclusion: the present report provides new information about the protein content of human neutrophils. Importantly, our study resulted in the discovery of a series of proteins not previously reported to be associated with human neutrophils. These data are relevant to the investigation of comparative pathological states and models for novel classes of pharmaceutical drugs that could be useful in the treatment of inflammatory disorders in which neutrophils participate. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Norwegian Research Council
dc.format.extent 9
dc.language.iso eng
dc.publisher Biomed Central Ltd
dc.relation.ispartof Proteome Science
dc.rights Acesso aberto
dc.title Proteomic analysis of total cellular proteins of human neutrophils en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Bergen
dc.description.affiliation Univ São Paulo, Ctr Quim Prot, Ctr Reg Hemoterapia, BR-14049900 Ribeirao Preto, Brazil
dc.description.affiliation Univ São Paulo, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, Lab Oncol Expt LIM 24, São Paulo, Brazil
dc.description.affiliation Univ Bergen, Gade Inst, Microbiol & Immunol Sect, Bergen, Norway
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, São Paulo, Brazil
dc.description.sponsorshipID Norwegian Research Council: 175141
dc.identifier.file WOS000270069600001.pdf
dc.identifier.doi 10.1186/1477-5956-7-32
dc.description.source Web of Science
dc.identifier.wos WOS:000270069600001



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account