Proteomic analysis of total cellular proteins of human neutrophils

Proteomic analysis of total cellular proteins of human neutrophils

Author Tomazella, Gisele G. Autor UNIFESP Google Scholar
Silva, Idalete da Google Scholar
Laure, Helen J. Google Scholar
Rosa, Jose C. Google Scholar
Chammas, Roger Google Scholar
Wiker, Harald G. Google Scholar
Souza, Gustavo A. de Google Scholar
Greene, Lewis J. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Bergen
Abstract Background: Neutrophils are the most abundant leukocytes in peripheral blood and represent one of the most important elements of innate immunity. Recent subcellular proteomic studies have focused on the identification of human neutrophil proteins in various subcellular membrane and granular fractions. Although there are relatively few studies dealing with the analysis of the total extract of human neutrophils, many biological problems such as the role of chemokines, adhesion molecules, and other activating inputs involved in neutrophil responses and signaling can be approached on the basis of the identification of the total cellular proteins.Results: Using gel-LC-MS/MS, 251 total cellular proteins were identified from resting human neutrophils. This is more than ten times the number of proteins identified by an initial proteome analysis of human neutrophils and almost five times the number of proteins identified by the first 2-DE map of extracts of rat polymorphonuclear leukocytes. Most of the proteins identified in the present study are well-known, but some of them, such as neutrophil-secreted proteins and centaurin beta-1, a cytoplasmic protein involved in the regulation of NF-kappa B activity, are described here for the first-time.Conclusion: the present report provides new information about the protein content of human neutrophils. Importantly, our study resulted in the discovery of a series of proteins not previously reported to be associated with human neutrophils. These data are relevant to the investigation of comparative pathological states and models for novel classes of pharmaceutical drugs that could be useful in the treatment of inflammatory disorders in which neutrophils participate.
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Norwegian Research Council
Grant number Norwegian Research Council: 175141
Date 2009-08-31
Published in Proteome Science. London: Biomed Central Ltd, v. 7, 9 p., 2009.
ISSN 1477-5956 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 9
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000270069600001

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