Efficient method for obtaining Lep(ob)/Lep(ob)-derived animal models using adipose tissue transplantations

Efficient method for obtaining Lep(ob)/Lep(ob)-derived animal models using adipose tissue transplantations

Author Barros, C. C. Autor UNIFESP Google Scholar
Almeida, S. S. Autor UNIFESP Google Scholar
Mori, M. A. Autor UNIFESP Google Scholar
Valero, V. B. Autor UNIFESP Google Scholar
Haro, A. S. Autor UNIFESP Google Scholar
Batista, E. C. Autor UNIFESP Google Scholar
Rosa, T. S. Google Scholar
Bacurau, Reury Frank Pereira Google Scholar
Wuertele, M. Autor UNIFESP Google Scholar
Araujo, R. C. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Mogi das Cuzes
Universidade de São Paulo (USP)
Abstract Background: Leptin-deficient mice (Lep(ob)/Lep(ob), also known as ob/ob) are of great importance for studies of obesity, diabetes and other correlated pathologies. Thus, generation of animals carrying the Lep(ob) gene mutation as well as additional genomic modifications has been used to associate genes with metabolic diseases. However, the infertility of Lep(ob)/Lep(ob) mice impairs this kind of breeding experiment.Objective: To propose a new method for production of Lep(ob)/Lep(ob) animals and Lep(ob)/Lep(ob)-derived animal models by restoring the fertility of Lep(ob)/Lep(ob) mice in a stable way through white adipose tissue transplantations.Methods: for this purpose, 1 g of peri-gonadal adipose tissue from lean donors was used in subcutaneous transplantations of Lep(ob)/Lep(ob) animals and a crossing strategy was established to generate Lep(ob)/Lep(ob)-derived mice.Results: the presented method reduced by four times the number of animals used to generate double transgenic models (from about 20 to 5 animals per double mutant produced) and minimized the number of genotyping steps (from 3 to 1 genotyping step, reducing the number of Lep gene genotyping assays from 83 to 6).Conclusion: the application of the adipose transplantation technique drastically improves both the production of Lep(ob)/Lep(ob) animals and the generation of Lep(ob)/Lep(ob)-derived animal models. International Journal of Obesity (2009) 33, 938-944; doi: 10.1038/ijo.2009.95; published online 16 June 2009
Keywords ob/ob
adipose tissue transplantation
breeding
leptin
fertility
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 06/590816
CAPES: PROCAD-0216/05-4
Date 2009-08-01
Published in International Journal of Obesity. London: Nature Publishing Group, v. 33, n. 8, p. 938-944, 2009.
ISSN 0307-0565 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent 938-944
Origin http://dx.doi.org/10.1038/ijo.2009.95
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000268799200017
URI http://repositorio.unifesp.br/handle/11600/31688

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account