Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion

Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion

Author Paiva, Vanessa Nunes de Autor UNIFESP Google Scholar
Monteiro, Rebecca M. M. Autor UNIFESP Google Scholar
Marques, Vilmar de Paiva Google Scholar
Cenedeze, Marcos Antonio Autor UNIFESP Google Scholar
Teixeira, Vicente de P. A. Google Scholar
Reis, Marlene A. dos Google Scholar
Pacheco-Silva, Alvaro Autor UNIFESP Google Scholar
Camara, Niels O. S. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Hosp Israelita Albert Einstein
Univ Fed Triangulo Mineiro
Abstract Renal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-gamma, and IFN-gamma/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. in each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric analyses. Gene expression of IL-6 and HO-1 were also investigated by Q-PCR. IFN-gamma KO animals presented the highest impairment in renal function compared to controls. Conversely, IL-12 KO animals were absolutely protected and, in a lesser extent, IFN-gamma/IL-12 KO double knockout was also protected from IRI. Gene expression analyses showed higher expression of HO-1, a cytoprotective gene, and IL-6, a pro-inflammatory cytokine, in IFN-gamma deficient animals subjected to IRI. Our results confirm that Th1 related cytokines such as IL-12 and IFN-gamma are critically involved in renal ischemia and reperfusion injury. (C) 2008 Elsevier B.V. All rights reserved.
Keywords Acute renal failure
Ischemia and reperfusion injury
Interferon gamma
Language English
Date 2009-06-01
Published in International Immunopharmacology. Amsterdam: Elsevier B.V., v. 9, n. 6, p. 668-672, 2009.
ISSN 1567-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 668-672
Access rights Closed access
Type Article
Web of Science ID WOS:000266568800005

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