Native LDL-Cholesterol Mediated Monocyte Adhesion Molecule Overexpression is Blocked by Simvastatin

Native LDL-Cholesterol Mediated Monocyte Adhesion Molecule Overexpression is Blocked by Simvastatin

Author Serrano, Carlos V. Google Scholar
Pesaro, Antonio Eduardo Google Scholar
Lemos, James A. de Google Scholar
Rached, Fabiana Google Scholar
Segre, C. Alexandre Google Scholar
Gomes, Fernando Google Scholar
Ribeiro, Adriano F. Google Scholar
Nicolau, Jose Carlos Google Scholar
Yoshida, Vanda M. Google Scholar
Monteiro, Hugo P. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Univ Texas SW Med Ctr Dallas
Universidade Federal de São Paulo (UNIFESP)
Abstract Aim of the study This study sought to evaluate the effect of nLDL concentrations on monocyte adhesion molecule expression in hypercholesterolemic patients with stable corollary artery disease (CAD) and to determine whether lipid-lowering therapy with simvastatin Would change this effect.Methods Blood samples from patients with hypercholesterolemia (mean LDL 152 mg/dL) and CAD (HC, n = 23) were collected before and after a 12-week treatment with 40 mg of simvastatin. Healthy individuals (mean LDL 111 mg/dL) were used as controls (CT, n = 15). Isolated nLDL, at a fixed concentration of 100 mg/dL, was added to monocyte suspensions obtained before and after the simvastatin treatment. Monocyte activation was determined by changes in cellular adhesion molecule expression.Results in response to nLDL, CD11b and CD14 adhesion molecule expression was higher in HC patients than in CT patients before treatment (174.2+/-8.4 vs 102.2+/-6.3, P<0.03 and 140.4+/-5.0 vs 90.4+/-6.7, P<0.04). After simvastatin treatment, CD11b expression decreased to 116.9+/-12.5 (P< 0.03) and CD14 expression to 107.5+/-6.2 (P<0.04). Alternatively, L-selectin expression was lower in HC patients than in CT patients before therapy (46.0+/-3.5 vs 62.1+/-5.5, P<0.04), and it increased markedly after lipid reduction to 58.7+/-5.0 (P<0.04 vs baseline). After simvastatin treatment, LDL was reduced to mean 101.5 mg/dL.Conclusions These data demonstrate that monocytes from HC patients are more prone to marked nLDL-mediated changes of adhesion molecule expression than monocytes from controls. Simvastatin is capable of inhibiting such nLDL effects. This proinflammatory response to nLDL may have a role in the early onset of atherosclerosis.
Keywords Native LDL-cholesterol
Adhesion molecules
Monocyte
Simvastatin
Atherosclerosis
Inflammation
Language English
Date 2009-06-01
Published in Cardiovascular Drugs and Therapy. Dordrecht: Springer, v. 23, n. 3, p. 215-220, 2009.
ISSN 0920-3206 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 215-220
Origin http://dx.doi.org/10.1007/s10557-008-6159-y
Access rights Closed access
Type Article
Web of Science ID WOS:000268200100007
URI http://repositorio.unifesp.br/handle/11600/31551

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