Ultrastructure of Motor Nerve Terminals in the Anterior Third of Wistar Rat Tongue

Ultrastructure of Motor Nerve Terminals in the Anterior Third of Wistar Rat Tongue

Author Motoyama, Aracy A. Google Scholar
Watanabe, Ii-Sei Google Scholar
Iyomasa, Mamie M. Google Scholar
Silva, Marcelo C. P. Autor UNIFESP Google Scholar
Sosthines, Marcia C. K. Google Scholar
Lopes, Marilia Gabriela O. Google Scholar
Guimaraes, J. P. Google Scholar
Kfoury, Jose R. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Univ Vale Paraiba
Abstract The nerve terminals of intrinsic muscular fibers of the tongue of adult wistar rats was studied by using silver impregnation techniques, transmission electron microscopy (TEM), and high resolution scanning electron microscopy (HRSEM) to observe the nerve fibers and their terminals. Silver impregnation was done according to Winkelman and Schmit, 1957. for TEM, small blocks were fixed in modified Karnovsky solution, postfixed in 1% buffered osmium tetroxide solution, and embedded in Spurr resin. for HRSEM, the parts were fixed in 2% osmium tetroxide solution with 1/15 M sodium phosphate buffer (pH 7.4) at 4 degrees C for 2 h, according to the technique described by Tanaka, 1989. Thick myelinated nerve bundles were histologically observed among the muscular fibers. the intrafusal nerve fiber presented a tortuous pathway with punctiform terminal axons in clusters contacting the surface of sarcolemma. Several myelinated nerve fibers involved by collagen fibers of the endoneurium were observed in HRSEM in three-dimensional aspects. the concentric lamellae of the myelin sheath and the axoplasm containing neurofilaments interspersed among the mitochondria were also noted. in TEM, myofibrils, mitochondria, rough endoplasmic reticulum, Golgi's apparatus, and glycogen granules were observed in sarcoplasm. It is also noted that the sarcomeres constituted by myofilaments with their A, I, and H bands and the electron dense Z lines. in areas adjacent to muscular fibers, there were myelinated and unmyelinated nerve fibers involved by endoneurium and perineurium. in the region of the neuromuscular junction, the contact with the sarcolemma of the muscular cell occurs forming several terminal buttons and showing numerous evaginations of the cell membrane. in the terminal button, mitochondria and numerous synaptic vesicles were observed. Microsc. Res. Tech. 72:464-470, 2009. (C) 2009 Wiley-Liss. Inc.
Keywords nerve fibers
mitochondria
vesicles
silver impregnation
tongue
rat
HRSEM
TEM
Language English
Date 2009-06-01
Published in Microscopy Research and Technique. Hoboken: Wiley-Blackwell, v. 72, n. 6, p. 464-470, 2009.
ISSN 1059-910X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 464-470
Origin http://dx.doi.org/10.1002/jemt.20692
Access rights Closed access
Type Article
Web of Science ID WOS:000267099100007
URI http://repositorio.unifesp.br/handle/11600/31540

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